Department of Medical Technology, School of Life and Environmental Science, Azabu University, Kanagawa, Japan
Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan.
Anticancer Res. 2021 Aug;41(8):4071-4076. doi: 10.21873/anticanres.15208.
BACKGROUND/AIM: Increased expression of inflammatory cytokine genes through cell interactions in tissues may cause chronic inflammation, leading to the development of lifestyle-related diseases. Since the activation of inflammatory cytokine genes in monocytes/macrophages by co-culturing with cancer cells or adipocytes was suppressed by pre-treatment with low-dose lipopolysaccharide (LPS), we hypothesized that low-dose LPS-activated macrophages may regulate the expression of immune response-related genes in other cells.
Phorbol myristate acetate-treated human monocytes (THP-1) were activated by LPS. The conditioned medium of LPS-activated THP-1 cells was added to human adipocytes. After 5 days, the expression of genes encoding interleukin (IL)-6 (IL6), IL-8 (IL8), monocyte chemotactic protein (MCP)-1 (CCL2), adiponectin (ADIPOQ), and plasminogen activator inhibitor (PAI)-1 (SERPINE1) was analyzed using quantitative real-time PCR.
The increased expression of inflammation-related genes and SERPINE1 in adipocytes was suppressed by the conditioned medium of THP-1 cells activated by low-dose LPS, whereas the expression of ADIPOQ was significantly increased.
Low-dose LPS-activated macrophages convert adipocytes to anti-inflammatory phenotypes.
背景/目的：细胞间相互作用导致组织中炎症细胞因子基因表达增加，可能引发慢性炎症，从而导致生活方式相关疾病的发生。由于低剂量脂多糖（LPS）预处理可抑制单核细胞/巨噬细胞中炎症细胞因子基因的激活，我们假设低剂量 LPS 激活的巨噬细胞可能调节其他细胞中免疫反应相关基因的表达。
用佛波醇肉豆蔻酸酯处理人单核细胞（THP-1），用 LPS 激活。将 LPS 激活的 THP-1 细胞的条件培养基加入人脂肪细胞中。5 天后，使用定量实时 PCR 分析编码白细胞介素（IL）-6（IL6）、IL-8（IL8）、单核细胞趋化蛋白（MCP）-1（CCL2）、脂联素（ADIPOQ）和纤溶酶原激活物抑制剂（PAI）-1（SERPINE1）的基因表达。
低剂量 LPS 激活的 THP-1 细胞的条件培养基可抑制脂肪细胞中炎症相关基因和 SERPINE1 的表达上调，而 ADIPOQ 的表达显著增加。
低剂量 LPS 激活的巨噬细胞可将脂肪细胞转化为抗炎表型。
