幼态持续和变态（甲状腺素诱导）的美西钝口螈（墨西哥钝口螈）大脑神经节苷脂的变化。

Changes in brain gangliosides of the neotene and metamorphic (thyroxine-induced) newt axolotl (Ambystoma mexicanum).

作者信息

Hilbig R, Schmitt M, Rahmann H

机构信息

Institute of Zoology, University of Stuttgart-Hohenheim, FRG.

出版信息

Dev Neurosci. 1987;9(4):240-6. doi: 10.1159/000111626.

DOI:10.1159/000111626

PMID:3428191

Abstract

Qualitative and quantitative changes in the concentration of proteins, sialoglycoproteins and gangliosides and in the composition of gangliosides in the brains of the neotene and the thyroxine-induced metamorphic newt axolotl (Ambystoma mexicanum) were investigated. During metamorphosis two polar gangliosides (GT1b and GQ1b) decreased by about 5% each. On the contrary GD1a increased to 10%. Another developmental trend was a slight increase of two other disialogangliosides (GD1b, GD2). Additionally, incorporation profiles (2-8 days) of 14C-N-Ac-mannosamine, the specific precursor for gangliosides, in the brain of neotene and metamorphic axolotls were followed giving evidence of significant changes in the sialoglycoconjugate metabolism of the central nervous system during metamorphosis of this newt.

摘要

研究了幼态持续和甲状腺素诱导变态的美西钝口螈（墨西哥钝口螈）大脑中蛋白质、唾液酸糖蛋白和神经节苷脂浓度的定性和定量变化以及神经节苷脂的组成。在变态过程中，两种极性神经节苷脂（GT1b和GQ1b）各自减少了约5%。相反，GD1a增加到10%。另一个发育趋势是另外两种二唾液酸神经节苷脂（GD1b、GD2）略有增加。此外，追踪了幼态持续和变态期美西钝口螈大脑中神经节苷脂的特异性前体14C-N-乙酰甘露糖胺的掺入情况（2-8天），这表明在这种蝾螈变态过程中，中枢神经系统的唾液酸糖缀合物代谢发生了显著变化。