13143The University of Tokyo, Tokyo, Japan.
FUJIFILM VET Systems Co, Ltd, Tokyo, Japan.
Vet Pathol. 2021 Nov;58(6):1033-1043. doi: 10.1177/03009858211030523. Epub 2021 Jul 20.
To elucidate the histopathological characteristics and immunophenotypes of canine transmural "mass-forming" gastrointestinal lymphomas and plasmacytomas, 83 surgically resected biopsy samples were examined. All lymphomas and plasmacytomas were located in the small or large intestine except for 1 plasmacytoma which was in the stomach. According to the World Health Organization (WHO) classification, B-cell neoplasms (17 cases) included lymphoplasmacytic lymphoma (6/17), plasmacytoma (5/17), follicular lymphoma (3/17), and diffuse large B-cell lymphoma (3/17). Based on nuclear sizes, T-cell neoplasms (66 cases) were broadly divided into large cell lymphoma (LCL; 48/66) and small cell lymphoma (SCL; 18/66). According to the WHO classification, T-cell neoplasms included anaplastic large T-cell lymphoma (ALCL; 10/66), angiotropic T-cell lymphoma (3/66), mixed inflammatory type peripheral T-cell lymphoma (mixed inflammatory type PTCL; 33/66), and PTCL-not otherwise specified (PTCL-NOS; 20/66). Mixed inflammatory type PTCLs were further divided into histiocyte- (27/33) and eosinophil- (6/33) dominant types. Immunohistochemically, lymphoplasmacytic lymphomas were positive for Pax5 (6/6) and IgM (5/6), while plasmacytomas were positive for IgG (5/6) and negative for Pax5. LCLs were immunopositive for granzyme B in 31/48 cases (65%) and CD8 in 9/48 cases (19%), while SCLs were immunopositive for granzyme B in 3/18 cases (17%) and CD8 in 3/18 cases (17%). Furthermore, 8/10 cases (80%) of ALCL and 19/27 cases (70%) of histiocyte-dominant PTCL were immunopositive for granzyme B, whereas 6/20 cases (30%) of PTCL-NOS, 1/6 cases (17%) of eosinophil-dominant PTCL, and no cases of angiotropic T-cell lymphomas were immunopositive for granzyme B. The present study describes the immunophenotypes in different histological types of transmural gastrointestinal lymphomas in the dog.
为了阐明犬“壁内”“肿块形成”胃肠道淋巴瘤和浆细胞瘤的组织病理学特征和免疫表型，检查了 83 例手术切除的活检样本。除了 1 例浆细胞瘤位于胃中外，所有的淋巴瘤和浆细胞瘤均位于小肠或大肠。根据世界卫生组织（WHO）分类，B 细胞肿瘤（17 例）包括淋巴浆细胞淋巴瘤（6/17）、浆细胞瘤（5/17）、滤泡淋巴瘤（3/17）和弥漫性大 B 细胞淋巴瘤（3/17）。根据核大小，T 细胞肿瘤（66 例）广泛分为大细胞淋巴瘤（LCL；48/66）和小细胞淋巴瘤（SCL；18/66）。根据 WHO 分类，T 细胞肿瘤包括间变性大细胞 T 细胞淋巴瘤（ALCL；10/66）、血管侵袭性 T 细胞淋巴瘤（3/66）、混合炎症型外周 T 细胞淋巴瘤（混合炎症型 PTCL；33/66）和未特指的 T 细胞淋巴瘤（PTCL-NOS；20/66）。混合炎症型 PTCL 进一步分为组织细胞（27/33）和嗜酸性粒细胞（6/33）优势型。免疫组化染色显示，淋巴浆细胞淋巴瘤对 Pax5（6/6）和 IgM（5/6）呈阳性，而浆细胞瘤对 IgG（5/6）呈阳性，对 Pax5 呈阴性。LCL 在 31/48 例（65%）中对 granzyme B 呈免疫阳性，在 9/48 例（19%）中对 CD8 呈免疫阳性，而 SCL 在 3/18 例（17%）中对 granzyme B 呈免疫阳性，在 3/18 例（17%）中对 CD8 呈免疫阳性。此外，10/10 例（80%）的 ALCL 和 27/27 例（70%）的组织细胞优势型 PTCL 对 granzyme B 呈免疫阳性，而 20/20 例（30%）的 PTCL-NOS、6/6 例（17%）的嗜酸性粒细胞优势型 PTCL 和没有血管侵袭性 T 细胞淋巴瘤对 granzyme B 呈免疫阳性。本研究描述了犬胃肠道壁内淋巴瘤不同组织学类型的免疫表型。
