Biology Department, College of Science, Mustansiriyah University, Baghdad, Iraq.
Mol Biol Rep. 2021 Jul;48(7):5393-5397. doi: 10.1007/s11033-021-06538-z. Epub 2021 Jul 20.
Cyanobacteria and their pollution are being increasingly commonly reported worldwide that cause a serious hazard to environmental and human health. Cyanotoxin was the most algal toxin reported to be produced by several orders of cyanobacteria. This study aimed to provide a technique to detect cylindrosprmopsin and saxitoxin biosynthesis genes in the river. In November, December 2019, and January 2020. Cyanobacteria were isolated from freshwater of Tigris River and identified by compound microscope also conventional PCR. Five isolates of cyanobacteria that successfully amplified a gene fragment from the phycocyanin were found in all cyanobacteria (Microcystis flosaquae, Microcystis sp, anabaena circinalis, nostoc commune and westiellopsis prolifica) and all isolates successfully amplified aoaC gene to detecting the cylidrospemopsin and the saxitoxin. Our results concluded that PCR assay can be used for early detection of cylidrospemopsin and the saxitoxin producing cyanobacteria in river water that useful to stations responsible for the preparation of drinking water to public.
蓝藻及其污染在全球范围内日益受到关注，对环境和人类健康造成严重危害。蓝藻毒素是由几种蓝藻产生的最常见的藻类毒素。本研究旨在提供一种检测河流中柱孢藻毒素和石房蛤毒素生物合成基因的技术。在 2019 年 11 月、12 月和 2020 年 1 月，从底格里斯河的淡水中分离出蓝藻，并通过复合显微镜和常规 PCR 进行鉴定。在所有蓝藻（微囊藻、微囊藻、鱼腥藻、念珠藻和西氏普雷沃氏菌）中都发现了 5 株成功从藻蓝蛋白扩增基因片段的蓝藻，所有分离株都成功扩增了 aoaC 基因，用于检测柱孢藻毒素和石房蛤毒素。我们的结果表明，PCR 检测可用于早期检测河水中产生柱孢藻毒素和石房蛤毒素的蓝藻，这对负责向公众供应饮用水的机构很有用。
