Postgraduation Program in Health and Environment, Universidade Metropolitana de Santos, Santos, SP, Brazil; Post Graduation Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil; Dentistry College, Universidade Metropolitana de Santos, Santos, SP, Brazil.
Postgraduation Program in Health and Environment, Universidade Metropolitana de Santos, Santos, SP, Brazil; Dentistry College, Universidade Metropolitana de Santos, Santos, SP, Brazil.
Photodiagnosis Photodyn Ther. 2021 Sep;35:102445. doi: 10.1016/j.pdpdt.2021.102445. Epub 2021 Jul 18.
This study aims to test the absorbance of a new composition of erythrosine, its pH, cell viability and potential as a photo sensitizer against Candida albicans when irratiaded with blue light emitting-diode (LED).
For pH and absorbance tests, erythrosine was prepared at a concentration of 0.03/ml. The cells of the L929 strain were cultured and the alamarBlue® assay was performed on samples to assess cell viability. For the microbiological essay, the strain of Candida albicans ATCC 90028 was selected. Yeast suspensions were divided into the following groups: control without irradiation or photosensitizer (C), irradiated group without photosensitizer (L), photosensitizer group without irradiation (0), and groups that received photosensitizer and irradiation, called aPDT groups.
Erythrosine had no significant changes in pH and its absorbance was also consistent (≅400 nm). When it came to cell viability, on the first day, the group that was in contact with the dye and irradiated with the LED in minimun power was found to have the higher cell proliferation. On day 3, both irradiated groups (maximum and minimum) showed the highest cell proliferation. In the microbiological essay with C. albicans, aPDT groups started to show microbial reduction after 60 and 90 s of irradiation and when irradiated for 120 s, 6 microbial reduction logs were found.
The erythrosine in question is a PS, with pH stability, blue light absorbance, cell viability and efficacy against C. albicans. More studies with this PS should be encouraged in order to verify its performance in aPDT.
本研究旨在测试一种新的赤藓红组成物的吸光度、pH 值、细胞活力以及在蓝光发光二极管（LED）照射下作为光增敏剂对抗白色念珠菌的潜力。
对于 pH 值和吸光度测试，将赤藓红制备成 0.03/ml 的浓度。培养 L929 株细胞，并对样品进行 alamarBlue® 检测以评估细胞活力。对于微生物学试验，选择白色念珠菌 ATCC 90028 株。将酵母悬浮液分为以下组：无照射或光敏剂的对照（C）、无照射光敏剂的照射组（L）、无照射光敏剂的光敏剂组（0）以及接受光敏剂和照射的组，称为 aPDT 组。
赤藓红的 pH 值没有明显变化，其吸光度也保持一致（≈400nm）。在细胞活力方面，第一天与染料接触并在最小功率下照射 LED 的组被发现具有更高的细胞增殖。第三天，两个照射组（最大和最小）显示出最高的细胞增殖。在白色念珠菌的微生物学试验中，aPDT 组在照射 60 和 90 秒后开始显示微生物减少，当照射 120 秒时，发现有 6 个微生物减少对数。
所研究的赤藓红是一种 PS，具有 pH 值稳定性、蓝光吸收、细胞活力和对抗白色念珠菌的功效。应该鼓励更多关于这种 PS 的研究，以验证其在 aPDT 中的性能。
