Moorman A F, Schalekamp M P, de Boer P A, Geerts W J, Lamers W H, Charles R
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
Differentiation. 1987;34(3):161-7. doi: 10.1111/j.1432-0436.1987.tb00063.x.
We used immunohistochemical procedures to investigate embryonic erythropoiesis in serial sections of chicken embryos after 2-13 days of incubation. Antibodies specific for the erythrocyte-specific histone H5, for embryonic hemoglobin, and for adult hemoglobin were used as markers for general, primitive, and definitive erythropoiesis, respectively. Histone H5 was present in erythrocytes at all of the stages studied, i.e., in both the primitive and definitive cells. Cell of the definitive lineage were first detected, at about 5-6 days of incubation, in erythroid foci in the mesenchyme around the vitelline stalk. At 7-9 days of incubation, a massive mesenchymal conglomeration of erythropoietic cells developed, extending from the cervical to the abdominal region and ventrally to the vertebral body, with its largest extensions being around the arteries in the mediastinum. Immunostaining revealed that these erythroid cells belonged to the definitive erythropoietic lineage. These cells had disappeared completely after 12 days of incubation, i.e., before erythropoiesis is visible in the bone marrow. These observations are consistent with the notion that the yolk sac is essential for the formation of the definitive erythroid lineage.
我们采用免疫组织化学方法，对孵化2至13天的鸡胚连续切片进行胚胎期红细胞生成研究。分别使用针对红细胞特异性组蛋白H5、胚胎血红蛋白和成人血红蛋白的抗体，作为总体、原始和定型红细胞生成的标志物。在所研究的所有阶段，即原始细胞和定型细胞中，红细胞均存在组蛋白H5。大约在孵化5至6天时，在卵黄蒂周围间充质中的类红细胞灶中首次检测到定型谱系的细胞。在孵化7至9天时，形成了大量造血细胞的间充质聚集，从颈部延伸至腹部区域，并向腹侧延伸至椎体，其最大延伸部分位于纵隔的动脉周围。免疫染色显示这些类红细胞属于定型造血谱系。在孵化12天后，即骨髓中可见红细胞生成之前，这些细胞已完全消失。这些观察结果与卵黄囊对定型红细胞谱系形成至关重要的观点一致。
