Mondal Milon, Hossen Md Sakib, Rahman Mohammad Asikur, Saha Sushmita, Sarkar Chandan, Bhoumik Nikhil Chandra, Kundu Sukalyan Kumar
Department of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, Bangladesh.
Department of Biochemistry, Primeasia University, Banani, 1213, Bangladesh.
Toxicol Rep. 2021 Jul 8;8:1369-1380. doi: 10.1016/j.toxrep.2021.07.003. eCollection 2021.
is a traditional medicinal plant for treating diverse ailments. Hence, we designed our study to scrutinize the protective effect of the methanol extract of leaf (BTL) against carbofuran-induced oxidative stress-mediated hepato-toxicity in Sprague-Dawley rats for the first time, along with the identification and quantification of phenolic acids and flavonoids by high-performance liquid chromatography (HPLC) and evaluation of antioxidant and antiradical activities of this extract. HPLC analysis confirmed the existence of tannic acid, gallic acid, salicylic acid, and naringin in leaf extract which showed in-vitro antioxidant potentialities with DPPH, nitric oxide, hydrogen peroxide, and hydroxyl radical scavenging properties. Co-administration of leaf extract with carbofuran showed dose-dependent significant protective effects of hepatic toxicity on serum markers such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ-glutamyl-transferase, lactate dehydrogenase, total bilirubin, total protein, albumin, globulin, lipid profile, urea, uric acid, and creatinine. Carbofuran intoxication also revealed an upsurge in malondialdehyde (MDA) and a decline in cellular endogenous antioxidant enzyme levels in rats compared with the control group. However, leaf extract co-treatment increased the levels of hepatic antioxidant enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase, and amended the MDA level. Similarly, histopathological evaluation further assured that BTL could keep the hepatocyte from carbofuran-induced damage. Therefore, all of our findings may conclude that the phenolic acids and flavonoids of leaf extract are responsible to neutralize the toxic free radical-mediated oxidative hepatic damages.
是一种用于治疗多种疾病的传统药用植物。因此，我们首次设计了本研究，以仔细研究叶（BTL）甲醇提取物对毒死蜱诱导的Sprague-Dawley大鼠氧化应激介导的肝毒性的保护作用，同时通过高效液相色谱（HPLC）鉴定和定量酚酸和黄酮类化合物，并评估该提取物的抗氧化和抗自由基活性。HPLC分析证实叶提取物中存在单宁酸、没食子酸、水杨酸和柚皮苷，这些物质在体外具有DPPH、一氧化氮、过氧化氢和羟基自由基清除特性的抗氧化潜力。叶提取物与毒死蜱共同给药对血清标志物如丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶、γ-谷氨酰转移酶、乳酸脱氢酶、总胆红素、总蛋白、白蛋白、球蛋白、血脂、尿素、尿酸和肌酐等肝毒性具有剂量依赖性的显著保护作用。与对照组相比，毒死蜱中毒还导致大鼠丙二醛（MDA）升高和细胞内源性抗氧化酶水平下降。然而，叶提取物联合处理可提高肝抗氧化酶如超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平，并改善MDA水平。同样，组织病理学评估进一步证实BTL可以防止肝细胞受到毒死蜱诱导的损伤。因此，我们所有的研究结果可能得出结论，叶提取物中的酚酸和黄酮类化合物负责中和有毒自由基介导的肝脏氧化损伤。
