Department of Pharmacy , Jahangirnagar University , Savar , Dhaka 1342 , Bangladesh.
Department of Pharmacy , Bangabandhu Sheikh Mujibur Rahman Science and Technology University , Gopalganj 8100 , Bangladesh.
Chem Res Toxicol. 2019 Aug 19;32(8):1619-1629. doi: 10.1021/acs.chemrestox.9b00164. Epub 2019 Jul 23.
The aim of the present study was to evaluate the protective effect of leaf methanol extract (SCL) against carbofuran (CF)-induced hepatotoxicity in Sprague-Dawley rats, along with the identification and quantification of polyphenolic composition by high-performance liquid chromatography (HPLC). Results revealed the presence of alkaloids, tannins, and flavonoids in SCL. Similarly, HPLC analysis suggests that SCL contains some known important antioxidants, such as rutin, benzoic acid, and salicylic acid that could be responsible for the hepatoprotective activity of the extract. In CF-exposed rats, significant hematological alterations along with histological changes were marked by the presence of necrosis, congestion, and inflammation. CF-intoxication also showed an increase in lipid peroxidation and decrease in cellular antioxidant enzymes (e.g., superoxide dismutase, catalase, and glutathione peroxidase) levels in rats compared with the control group. Furthermore, coadministration of SCL significantly ameliorated the abnormalities and improved the cellular arrangement in experimental animals. SCL also reversed the alteration of hematological and biochemical parameters and brought them back to normal levels as compared to the control group. In conclusion, may be one of the best sources of natural antioxidant compounds that can be used in the treatment of oxidative stress and stress-related diseases and disorders.
本研究旨在评估叶甲醇提取物（SCL）对 Sprague-Dawley 大鼠卡巴呋喃（CF）诱导的肝毒性的保护作用，并通过高效液相色谱法（HPLC）鉴定和定量多酚组成。结果表明 SCL 中存在生物碱、单宁和类黄酮。同样，HPLC 分析表明 SCL 含有一些已知的重要抗氧化剂，如芦丁、苯甲酸和水杨酸，这些抗氧化剂可能是提取物具有保肝活性的原因。在 CF 暴露的大鼠中，存在坏死、充血和炎症，表明血液学发生了显著变化，组织学也发生了变化。与对照组相比，CF 中毒还导致大鼠脂质过氧化增加，细胞抗氧化酶（如超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）水平降低。此外，SCL 的共同给药显著改善了实验动物的异常情况，并改善了细胞排列。与对照组相比，SCL 还逆转了血液学和生化参数的改变，并使其恢复正常水平。总之，SCL 可能是天然抗氧化化合物的最佳来源之一，可用于治疗氧化应激和与应激相关的疾病和障碍。
