Chen Fei, Wegner Seraphine V
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
Bio Protoc. 2018 Jun 20;8(12):e2893. doi: 10.21769/BioProtoc.2893.
Control of bacterial adhesions to a substrate with high precision in space and time is important to form a well-defined biofilm. Here, we present a method to engineer bacteria such that they adhere specifically to substrates under blue light through the photoswitchable proteins nMag and pMag. This provides exquisite spatiotemporal remote control over these interactions. The engineered bacteria express pMag protein on the surface so that they can adhere to substrates with nMag protein immobilization under blue light, and reversibly detach in the dark. This process can be repeatedly turned on and off. In addition, the bacterial adhesion property can be adjusted by expressing different pMag proteins on the bacterial surface and altering light intensity. This protocol provides light switchable, reversible and tunable control of bacteria adhesion with high spatial and temporal resolution, which enables us to pattern bacteria on substrates with great flexibility.
在空间和时间上高精度控制细菌与底物的粘附对于形成明确的生物膜很重要。在此，我们提出一种改造细菌的方法，使它们通过光开关蛋白nMag和pMag在蓝光下特异性粘附到底物上。这提供了对这些相互作用的精确时空远程控制。工程细菌在表面表达pMag蛋白，以便它们在蓝光下能与固定有nMag蛋白的底物粘附，并在黑暗中可逆地分离。这个过程可以反复开启和关闭。此外，通过在细菌表面表达不同的pMag蛋白并改变光强度，可以调节细菌的粘附特性。该方案提供了具有高空间和时间分辨率的光控、可逆且可调的细菌粘附控制，这使我们能够非常灵活地在底物上对细菌进行图案化。
