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本文引用的文献

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Non-Neuronal Cells Are Required to Mediate the Effects of Neuroinflammation: Results from a Neuron-Enriched Culture System.介导神经炎症效应需要非神经元细胞：来自富含神经元的培养系统的结果。
PLoS One. 2016 Jan 20;11(1):e0147134. doi: 10.1371/journal.pone.0147134. eCollection 2016.
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Striatal medium-sized spiny neurons: identification by nuclear staining and study of neuronal subpopulations in BAC transgenic mice.纹状体中型多棘神经元：通过核染色鉴定及对BAC转基因小鼠中神经元亚群的研究
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Cortico-striatal synaptic defects and OCD-like behaviours in Sapap3-mutant mice.Sapap3基因敲除小鼠的皮质-纹状体突触缺陷及类强迫症行为
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The frequency and distribution of medium-sized neurons with indented nuclei in the primate and rodent neostriatum.
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Differential loss of striatal projection neurons in Huntington disease.亨廷顿病中纹状体投射神经元的差异性丢失
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小鼠胚胎纹状体神经元的分离与培养

Isolation and Maintenance of Murine Embryonic Striatal Neurons.

作者信息

Naia Luana, Rego A Cristina

机构信息

CNC-Center for Neuroscience and Cell Biology, University of Coimbra (UC), Coimbra, Portugal.

FMUC - Faculty of Medicine, UC, Coimbra, Portugal.

出版信息

Bio Protoc. 2018 Apr 20;8(8):e2823. doi: 10.21769/BioProtoc.2823.

DOI:10.21769/BioProtoc.2823
PMID:34286033
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8275303/
Abstract

Primary cultures of murine striatal neurons are widely used to explore cellular mechanisms in neurobiology, including brain diseases. Here we describe a detailed and standardized protocol to dissect and culture embryonic murine striatal neurons GABA-positive/DARPP-32-positive for 12 days , when they show good neuronal cell connectivity and the presence of dendritic spines, which reflects the maturation of the network.

摘要

小鼠纹状体神经元的原代培养被广泛用于探索神经生物学中的细胞机制，包括脑部疾病。在此，我们描述了一种详细且标准化的方案，用于解剖和培养胚胎小鼠纹状体GABA阳性/DARPP - 32阳性神经元12天，此时它们表现出良好的神经元细胞连接性以及树突棘的存在，这反映了网络的成熟。