前体供应增加了 NRPS-PKS 基因表达后 4-羟基-6-(4-羟基苯基)-α-吡喃酮的积累。
Precursor Supply Increases the Accumulation of 4-Hydroxy-6-(4-hydroxyphenyl)-α-pyrone after NRPS-PKS Gene Expression.
机构信息
Institut für Pharmazeutische Biologie und Biotechnologie, Fachbereich Pharmazie, Philipps-Universität Marburg, Robert-Koch Straße 4, 35037 Marburg, Germany.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, People's Republic of China.
出版信息
J Nat Prod. 2021 Aug 27;84(8):2380-2384. doi: 10.1021/acs.jnatprod.1c00120. Epub 2021 Jul 21.
Expression of a nonribosomal peptide synthetase-nonreducing polyketide synthase hybrid gene from PRB-2 in led to the accumulation of 4-hydroxy-6-(4-hydroxyphenyl)-α-pyrone (). Adding -hydroxybenzoic acid into the medium in which the overexpressing mutant is growing increased the product yield up to 5-fold. This strategy could be helpful for heterologous gene expression experiments requiring special substrates for product formation.
表达来自 PRB-2 的非核糖体肽合酶-非还原型聚酮合酶杂合基因导致了 4-羟基-6-(4-羟基苯基)-α-吡喃酮 () 的积累。在过表达突变体生长的培养基中添加 -羟基苯甲酸可将产物产量提高 5 倍。该策略对于需要特殊底物用于产物形成的异源基因表达实验可能会有所帮助。
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