羟胺诱变作用的机制 XIII. 在羟胺存在下噬菌体 MS2 琥珀突变体的回复突变
The mechanism of the mutagenic action of hydroxylamine XIII. Reversion of phage MS2 amber mutants in the presence of hydroxylamines.
作者信息
Budowsky E I, Klebanova L M, Metlitskaya A Z
出版信息
Mutat Res. 1978 Feb;49(2):173-8. doi: 10.1016/0027-5107(78)90154-9.
The replication of the phage MS2 in the presence of either hydroxylamine (HA) or O-methylhydroxylamine (OMHA) (mutagenesis in vivo) results in an increase in the reversion frequency of two amber mutations in the maturation protein. When acting on the extracellular phage (mutagenesis in vitro) the mutagens do not affect the reversion frequency. The most probable mode of mutagenic action of the hydroxylamines on the vegetative MS2 phage involves the enzymic formation of modified precursors and their incorporation into RNA.
在存在羟胺（HA）或O - 甲基羟胺（OMHA）（体内诱变）的情况下，噬菌体MS2的复制导致成熟蛋白中两个琥珀突变的回复频率增加。当作用于细胞外噬菌体（体外诱变）时，诱变剂不影响回复频率。羟胺对营养型MS2噬菌体的诱变作用最可能的模式涉及修饰前体的酶促形成及其掺入RNA。
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