Department of Chemistry, Wuhan University, Wuhan, China.
Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, China.
Rapid Commun Mass Spectrom. 2021 Oct 15;35(19):e9169. doi: 10.1002/rcm.9169.
3-Monochloropropane-1,2-diol (3-MCPD) is a well-known contaminant formed in food thermal processing, which could be found in a variety of foodstuffs. Due to its potential carcinogenicity, it was essential to quickly develop a rapid and high-throughput analytical method to monitor 3-MCPD in foodstuffs, which is described in this study.
3-MCPD was extracted from foodstuffs and then was derivatized with a boronic acid-modified C (B-C ) through the boronic acid-diol reaction. Microwave heating was used to accelerate the derivatization reaction. Mass spectrometry (MS) analysis was conducted using matrix-assisted laser desorption/ionization-MS (MALDI-MS). The application of the method was validated using various smoked food samples.
The chemical derivatization of 3-MCPD with B-C enabled the addition of a C -tag to 3-MCPD. High-throughput analysis of the sample within 0.5 h was realized. A good linear range from 0.02 to 1.5 μg mL for 3-MCPD was obtained, with a detection limit of 0.005 μg mL . The recoveries in spiked foodstuffs ranged from 85.4% to 115.1% with relative standard deviations of 2.0%-14.2%. This method was successfully applied to detect 3-MCPD in smoked foodstuffs.
A quantitative method was developed for the detection of 3-MCPD in foodstuffs using B-C derivatization combined with MALDI-MS strategy. This proposed method may serve as a potential platform for the rapid and high-throughput analysis of 3-MCPD in foodstuffs for the purpose of food safety control.
3-单氯丙二醇（3-MCPD）是一种在食品热加工过程中形成的知名污染物，存在于多种食品中。由于其潜在的致癌性，快速开发一种快速、高通量的分析方法来监测食品中的 3-MCPD 至关重要，本研究即对此进行了描述。
3-MCPD 从食品中提取出来，然后通过硼酸-二醇反应与硼酸修饰的 C（B-C）衍生化。微波加热用于加速衍生化反应。采用基质辅助激光解吸/电离-MS（MALDI-MS）进行质谱（MS）分析。该方法通过各种熏制食品样品进行了验证。
B-C 对 3-MCPD 的化学衍生化使 3-MCPD 加上了一个 C-标记。在 0.5 小时内实现了对样品的高通量分析。3-MCPD 的线性范围良好，为 0.02 至 1.5 μg/mL，检测限为 0.005 μg/mL。在添加的食品中的回收率在 85.4%至 115.1%之间，相对标准偏差为 2.0%-14.2%。该方法成功应用于检测熏制食品中的 3-MCPD。
采用 B-C 衍生化结合 MALDI-MS 策略，建立了一种用于检测食品中 3-MCPD 的定量方法。该方法可作为食品安全控制目的的食品中 3-MCPD 快速高通量分析的潜在平台。
