Kodama H, Yamada F, Kurosawa T, Mikami T, Izawa H
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Appl Bacteriol. 1987 Sep;63(3):255-60. doi: 10.1111/j.1365-2672.1987.tb04944.x.
The amount of endotoxin in serum collected from normal rainbow trout (Salmo gairdneri) and trout inoculated with viable Vibrio anguillarum or lipopolysaccharide (LPS) extracted from bacteria was determined by the chromogenic substrate method. The mean values of endotoxin in four different groups of normal rainbow trout sera ranged from 31.9 to 65.3 pg/ml. When fish were inoculated with viable bacteria (1 x 10(8], they became septicaemic and a large amount of endotoxin ng/ml) was detected in the sera. In fish inoculated with a smaller number of bacteria the amount of endotoxin was several times higher than that of normal fish in spite of failure of bacterial isolation. Although the endotoxin level in serum increased rapidly (greater than 100 ng/ml) after intraperitoneal inoculation with purified V. anguillarum LPS (540 micrograms), no fish died during the experiment. The high level of endotoxin in normal rainbow trout and the resistance of trout to endotoxin are in striking contrast to those of mammalian and avian species.
采用显色底物法测定了从正常虹鳟（鲑鱼）以及接种了活鳗弧菌或从细菌中提取的脂多糖（LPS）的虹鳟血清中的内毒素含量。四组不同的正常虹鳟血清内毒素平均值在31.9至65.3 pg/ml之间。当鱼接种活细菌（1×10⁸）时，它们会发生败血症，并且在血清中检测到大量内毒素（ng/ml）。在接种较少数量细菌的鱼中，尽管未能分离出细菌，但内毒素含量比正常鱼高出数倍。尽管腹腔注射纯化的鳗弧菌LPS（540微克）后血清内毒素水平迅速升高（大于100 ng/ml），但实验期间没有鱼死亡。正常虹鳟中内毒素的高水平以及虹鳟对内毒素的抗性与哺乳动物和鸟类形成了鲜明对比。
