Righezza M, Murello M H, Siouffi A M
Laboratoire de Chimie Appliquée et Génie Chimique, Université Aix-Marseille III, France.
J Chromatogr. 1987 Nov 20;410(1):145-55. doi: 10.1016/s0021-9673(00)90042-6.
The liquid chromatographic detection of nitrosamines at the ppb (10(9)) and sub-ppb levels is still a difficult problem. A significant improvement was achieved when a nitrosamine eluted from the column was photolysed by a UV lamp, the charged nitrite species being detected amperometrically. N-Nitrosodimethylamine (NDMA) was selected as a model solute to test the applicability of the detection device. The calibration graphs were linear over a wide concentration range. Optimal detection depended on the nature of the buffer used and the UV spectrum of the nitrosamine to be detected. An interesting application of the detection of a high-molecular-weight nitrosamine is given as an example.
在十亿分之一（10⁻⁹）和亚十亿分之一水平下对亚硝胺进行液相色谱检测仍然是一个难题。当从柱中洗脱出来的亚硝胺被紫外灯光解，带电的亚硝酸盐物种通过安培法进行检测时，取得了显著的改进。选择N-亚硝基二甲胺（NDMA）作为模型溶质来测试检测装置的适用性。校准曲线在很宽的浓度范围内呈线性。最佳检测取决于所用缓冲液的性质以及待检测亚硝胺的紫外光谱。给出了一个检测高分子量亚硝胺的有趣应用实例。
