Suppr超能文献

一种水提取物的体外和体内遗传毒性评估。

Evaluation of the in vitro and in vivo genotoxicity of a water extract.

作者信息

Cha Seung-Beom, Kim Seong-Sook, Oh Jeong-Ja, Lee Woo-Joo, Song Si-Whan, Lim Je-Oh, Kim Jong-Choon

机构信息

Nonclinical Research Center, ChemOn Inc., Yongin, 17162 Korea.

College of Veterinary Medicine (BK21 Plus Project Team), Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186 Korea.

出版信息

Toxicol Res. 2021 Jan 2;37(3):385-393. doi: 10.1007/s43188-020-00077-8. eCollection 2021 Jul.

Abstract

is commonly used in traditional herbal medicines for the treatment of diabetes, hyperthyroidism, liver damage, neuropathy, and asthma. Here, we investigated the genotoxicity potential of water extract (DRWE) using three standard battery systems in accordance with the test guidelines of the Organisation for Economic Cooperation and Development and Ministry of Food and Drug Safety as well as the principles of Good Laboratory Practice. A bacterial reverse mutation test (Ames test) was performed using the direct plate incorporation method in the presence or absence of a metabolic activation system (S9 mixture). The tester strains used included four histidine auxotrophic strains of , TA100, TA1535, TA98, and TA1537, along with a tryptophan auxotrophic strain of , WP2 A. An in vitro chromosome aberration test was performed using CHL/IU cells originally derived from the lung of a female Chinese hamster in the presence or absence of the S9 mixture. An in vivo mouse bone marrow micronucleus test was performed using male ICR mice. The micronucleus was confirmed after observation of the micro-nucleated polychromatic. The Ames test showed that DRWE did not induce gene mutations at any dose level in any of the tested strains. Additionally, DRWE did not result in any chromosomal aberrations specified in the in vitro chromosomal aberration and in vivo micronucleus tests. These results showed that DRWE exhibited neither mutagenic nor clastogenic potential in either the in vitro or in vivo test systems.

摘要

常用于传统草药中治疗糖尿病、甲状腺功能亢进、肝损伤、神经病变和哮喘。在此,我们根据经济合作与发展组织以及食品药品安全部的测试指南以及良好实验室规范原则,使用三种标准检测系统研究了水提取物(DRWE)的遗传毒性潜力。采用直接平板掺入法在有或无代谢活化系统(S9混合物)的情况下进行细菌回复突变试验(Ames试验)。所使用的测试菌株包括四种组氨酸营养缺陷型菌株,TA100、TA1535、TA98和TA1537,以及一种色氨酸营养缺陷型菌株,WP2 A。在有或无S9混合物的情况下,使用最初源自中国雌性仓鼠肺的CHL/IU细胞进行体外染色体畸变试验。使用雄性ICR小鼠进行体内小鼠骨髓微核试验。在观察到含微核的多色红细胞后确认微核。Ames试验表明,DRWE在任何测试菌株的任何剂量水平下均未诱导基因突变。此外,在体外染色体畸变试验和体内微核试验中,DRWE均未导致任何特定的染色体畸变。这些结果表明,DRWE在体外或体内测试系统中均未表现出诱变或断裂潜力。

相似文献

1
Evaluation of the in vitro and in vivo genotoxicity of a water extract.
Toxicol Res. 2021 Jan 2;37(3):385-393. doi: 10.1007/s43188-020-00077-8. eCollection 2021 Jul.
2
Genotoxicity evaluation of a Phragmitis rhizoma extract using a standard battery of in vitro and in vivo assays.
J Ethnopharmacol. 2019 Sep 15;241:112025. doi: 10.1016/j.jep.2019.112025. Epub 2019 Jun 9.
3
In vitro and in vivo evaluation of the genotoxicity of Eriobotrya japonica leaf extract.
Regul Toxicol Pharmacol. 2018 Nov;99:238-243. doi: 10.1016/j.yrtph.2018.09.029. Epub 2018 Sep 27.
4
Evaluation of oral toxicity and genotoxicity of Achyranthis Radix extract.
J Ethnopharmacol. 2021 Jun 28;274:113944. doi: 10.1016/j.jep.2021.113944. Epub 2021 Mar 9.
6
Genotoxicity evaluation of Hwanglyeonhaedok-tang, an herbal formula.
J Ethnopharmacol. 2017 Apr 18;202:122-126. doi: 10.1016/j.jep.2016.11.051. Epub 2016 Dec 1.
7
In vitro and in vivo genotoxicity tests on fullerene C60 nanoparticles.
Toxicol Lett. 2009 Dec 15;191(2-3):289-96. doi: 10.1016/j.toxlet.2009.09.012. Epub 2009 Sep 20.
8
Aqueous extract of Forsythia viridissima fruits: Acute oral toxicity and genotoxicity studies.
J Ethnopharmacol. 2020 Mar 1;249:112381. doi: 10.1016/j.jep.2019.112381. Epub 2019 Nov 9.
9
Evaluation of genetic toxicity of 6-diazo-5-oxo-l-norleucine (DON).
Toxicol Mech Methods. 2017 Sep;27(7):518-527. doi: 10.1080/15376516.2017.1333552. Epub 2017 Jun 20.
10
Evaluation of genotoxicity of SUNACTIVE Zn-P240 in vitro and in vivo.
Toxicol Res. 2022 Mar 26;38(4):459-467. doi: 10.1007/s43188-022-00128-2. eCollection 2022 Oct.

引用本文的文献

1
Acute toxicity and genotoxicity studies on new melatonergic antidepressant GW117.
Heliyon. 2023 Feb 24;9(3):e14026. doi: 10.1016/j.heliyon.2023.e14026. eCollection 2023 Mar.

本文引用的文献

1
Recommended criteria for the evaluation of bacterial mutagenicity data (Ames test).
Mutat Res Genet Toxicol Environ Mutagen. 2019 Dec;848:403074. doi: 10.1016/j.mrgentox.2019.07.004. Epub 2019 Aug 5.
3
Genotoxicity Study of Glycopeptide (G-7%NANA).
Toxicol Res. 2018 Jul;34(3):259-266. doi: 10.5487/TR.2018.34.3.259. Epub 2018 Jul 15.
4
Evaluation of and genotoxicity of in a standard battery of assays.
Lab Anim Res. 2017 Sep;33(3):231-236. doi: 10.5625/lar.2017.33.3.231. Epub 2017 Sep 27.
5
The various aspects of genetic and epigenetic toxicology: testing methods and clinical applications.
J Transl Med. 2017 May 22;15(1):110. doi: 10.1186/s12967-017-1218-4.
6
Is the safety of herbal medicines for kidneys under question?
J Nephropharmacol. 2013 Jul 1;2(2):11-12. eCollection 2013.
8
Effects of Dioscoreae Rhizoma (SanYak) on Peripheral Neuropathy and its Safety.
J Pharmacopuncture. 2013 Sep;16(3):7-10. doi: 10.3831/KPI.2013.16.015.
9
Antihypertensive action of allantoin in animals.
Biomed Res Int. 2014;2014:690135. doi: 10.1155/2014/690135. Epub 2014 Mar 12.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验