Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, P.R. China.
ACS Appl Mater Interfaces. 2021 Aug 4;13(30):35561-35567. doi: 10.1021/acsami.1c10173. Epub 2021 Jul 23.
Adenosine triphosphate (ATP) is a small but significant biological molecule that plays an important role in regulating cellular metabolism processes. Accurate and sensitive analytical techniques for detecting ATP are urgently needed. Herein, a new electrochemical aptasensor was designed in light of DNA-templated copolymers that parallel to the electrode for sensitive gauging of ATP. The ATP aptamer decorated by the electronic medium ferrocene can be regarded as a ″bridge″ connecting two DNA-templated copolymers. When ATP exists, because of the extraordinary binding selectivity of DNA-templated copolymers for target ATP, the rapid electron transfer of ferrocene was beneficial to the sensitive detection of target ATP. Specifically, our parallel DNA copolymers are more stable than upright DNA copolymers and have a faster signal transmission because of the close distance to the electrode; meanwhile, the nonspecific pollution is less. Consequently, the developed novel aptasensor exhibited a wide range of linear response toward ATP that was from 0.1 to 10 000 pM and high selectivity with a detection limit down to 11.5 fM. The electrochemical aptasensor has a broad application prospect in the detection of small biological molecules.
三磷酸腺苷（ATP）是一种体积虽小但意义重大的生物分子，在调节细胞代谢过程中起着重要作用。因此，我们迫切需要准确、灵敏的分析技术来检测 ATP。在此，我们设计了一种新的电化学适体传感器，基于平行于电极的 DNA 模板共聚物，用于灵敏检测 ATP。由电子媒介二茂铁修饰的 ATP 适体可以被视为连接两个 DNA 模板共聚物的“桥梁”。当存在 ATP 时，由于 DNA 模板共聚物对靶标 ATP 具有非凡的结合选择性，二茂铁的快速电子转移有利于靶标 ATP 的灵敏检测。具体来说，我们的平行 DNA 共聚物比直立 DNA 共聚物更稳定，由于与电极的距离更近，信号传输更快；同时，非特异性污染也较少。因此，所开发的新型适体传感器对 ATP 表现出广泛的线性响应范围，从 0.1 到 10000 pM，并且具有很高的选择性，检测限低至 11.5 fM。电化学适体传感器在检测小分子生物分子方面具有广阔的应用前景。
