[子痫前期胎盘氨基酸转运活性的变化（使用人胎盘微绒毛膜囊泡）]
[The changes in EPH gestosis placental amino acid transport activity (using human placental microvillous membrane vesicles)].
作者信息
Iioka H, Moriyama I, Akazaki M, Itoh K, Hino K, Okamura Y, Itani Y, Katoh Y, Ichijo M
机构信息
Department of Obstetrics and Gynecology, Nara Medical University.
出版信息
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Oct;39(10):1745-8.
To elucidate the change in EPH gestosis placental amino acid transport activity, we investigated the uptake of L-alanine into microvillous membrane vesicles prepared from EPH gestosis placenta and from normal placenta by using a rapid filtration technique. 1. Alkaline phosphatase (ALP) was the marker enzyme of microvillous membrane vesicles (MMV). The ALP activity of mild EPH gestosis placental MMV didn't differ from that of normal placental MMV. On the other hand, the ALP activity of severe EPH gestosis placental MMV decreased compared to that of normal placental MMV. 2. The uptake of L-alanine into human placental MMV was dependent on the Na+ electrochemical gradient, so the transport across human placental MMV was a secondarily active one. The L-alanine transport activity of mild EPH gestosis placental MMV didn't differ from that of normal placental MMV. On the other hand, the L-alanine transport activity of severe EPH gestosis placental MMV decreased prominently compared to that of normal placental MMV.
为了阐明早发性子痫前期（EPH）胎盘氨基酸转运活性的变化，我们采用快速过滤技术，研究了L-丙氨酸进入从EPH子痫前期胎盘和正常胎盘制备的微绒毛膜囊泡的摄取情况。1. 碱性磷酸酶（ALP）是微绒毛膜囊泡（MMV）的标志酶。轻度EPH子痫前期胎盘MMV的ALP活性与正常胎盘MMV的ALP活性无差异。另一方面，重度EPH子痫前期胎盘MMV的ALP活性与正常胎盘MMV相比降低。2. L-丙氨酸进入人胎盘MMV的摄取依赖于Na+电化学梯度，因此跨人胎盘MMV的转运是一种继发性主动转运。轻度EPH子痫前期胎盘MMV的L-丙氨酸转运活性与正常胎盘MMV的无差异。另一方面，重度EPH子痫前期胎盘MMV的L-丙氨酸转运活性与正常胎盘MMV相比显著降低。
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