用于微血管同时明场和荧光成像的多光谱活体显微镜。
Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature.
作者信息
Janssen Barry G H, Najiminaini Mohamadreza, Zhang Yan Min, Omidi Parsa, Carson Jeffrey J L
机构信息
Department of Medical Biophysics, Western University, London, ON, N6A 5C1, Canada.
Kidney Clinical Research Unit (KCRU), London Health Sciences Centre, London, ON, N6C 6B5, Canada.
出版信息
Appl Microsc. 2021 Jul 24;51(1):12. doi: 10.1186/s42649-021-00059-6.
Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.
活体视频显微镜术可用于观察微循环血流。这通常需要荧光探针来可视化传统明场显微镜无法观察到的结构和动态过程。传统光学显微镜不允许同时进行明场和荧光成像。此外，在传统显微镜中，只能观察到一种类型的荧光标记。本研究引入了多光谱活体视频显微镜术，它在标准光学显微镜中结合了明场和荧光显微镜。该技术能够同时实时观察荧光标记结构及其直接物理环境。这一进展为微循环中标记结构的定位、运动和功能提供了背景信息。
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