Sedov V I
Zh Mikrobiol Epidemiol Immunobiol. 1978(12):52-5.
The most stable differential signs of enterococci are: growth in the medium at pH 10.2, growth in broth containing 40% bile, citrate utilization, resistance to 0,05% potassium tellurite, 2, 3, 5-triphenyltetrazolium chloride (TTC) reduction, the staining of colonies (plaques) on a medium with manganese, iron and zinc salts, glycerine fermentation under anaerobic conditions, mannite fermentation, the presence of hemolysin, of the proteolytic enzyme, and mobility. Combined differential-diagnostic nutrient medium permits to determine simultaneously 5 enterococci signs--resistance to nalidixic acid and to crystal violet, TTC reduction, hemolytic and proteolytic activity. The suggested scheme of enterococci laboratory diagnosis including a set of hard nutrient media poured into multisection Petri dish is simple reliable and accessible for any bacteriological laboratory.
在pH 10.2的培养基中生长、在含40%胆汁的肉汤中生长、利用柠檬酸盐、对0.05%亚碲酸钾有抗性、还原2,3,5-氯化三苯基四氮唑(TTC)、在含有锰、铁和锌盐的培养基上菌落(菌斑)的染色、厌氧条件下甘油发酵、甘露醇发酵、存在溶血素、蛋白水解酶以及运动性。组合鉴别诊断营养培养基可同时测定5种肠球菌特征——对萘啶酸和结晶紫的抗性、TTC还原、溶血和蛋白水解活性。所建议的肠球菌实验室诊断方案包括一套倒入多隔培养皿中的强化营养培养基,简单可靠,任何细菌学实验室均可采用。