Pregnancy and parturition in dromedary camels II. Incidence, timing and factors affecting early pregnancy loss (EPL) and the outcome of twin pregnancies.

In this paper, we described the incidence of early pregnancy loss (EPL) both after natural mating and embryo transfer, evaluated risk factors, and summarized the outcome of twin pregnancies throughout gestation in dromedaries under reproductive care. Data were collected over seven breeding seasons at the world's largest camel dairy farm (study 1). In addition, we determined the timing of EPL and monitored serum progesterone (P4) concentration between Days 13-70 of gestation during one breeding season (study 2). In the first study, out of 2970 pregnancies, 507 cases (17.1%) of EPL were diagnosed with transrectal ultrasonography. The rate of EPL after natural mating and embryo transfer was 16.1% (n = 422 out of 2616) and 24.0% (n = 85 out of 354), respectively. Twin pregnancies were detected in 215 cases (7.2% of all gestations), and 57 of those (26.5%) underwent complete EPL. Almost half of the early losses (n = 243; 47.9%) occurred before 30 d of gestation. Another 43.2% (n = 219) of EPL was diagnosed during the next month, and 8.9% (n = 45) occurred after 60 d of gestation. Multivariable mixed effects logistic regression models revealed that the breeding season (year) and twin pregnancy were the most important exposure variables affecting the rate of EPL (P < 0.001). The effect of some male camels was also demonstrated while other factors, such as type of breeding, age category, month of mating, breed/ecotype and reproductive history did not prove to have a significant influence. In the second study, the overall rate of EPL was 24.5% (n = 34 of 139). There was no difference in the incidence of EPL between ET recipient (24.2%, n = 23 of 95) and mated (25%, n = 11 of 44) camels. Weekly rate of EPL ranged from 0.9% to 4.8% with a decreasing tendency, and approx. 41% of the animals (n = 14 of 34) had some ultrasonographic signs of impending EPL 1 week before the final diagnosis. Mean serum P4 concentration in camels with subsequent EPL was 5.3 ± 0.1 ng/ml compared to 5.6 ± 0.04 ng/ml in normal pregnant dromedaries. Day of gestation and future EPL influenced serum P4 levels (P < 0.001) with an interaction between the two fixed factors (P < 0.05). At the time of the final diagnosis of EPL, mean serum P4 concentration was 2.8 ± 0.44 ng/ml. Although twinning had an unfavorable prognosis with a total pregnancy loss of 36.7%, it was not entirely detrimental for the final outcome of gestation as two-thirds of twin pregnancies (n = 136 out of 212) resulted in the birth of a live calf.