Liu Xiao, Liu Hongbing, Li Chunhua
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2021 Jul;35(7):658-661. doi: 10.13201/j.issn.2096-7993.2021.07.019.
Traditional conventional bacterial culture techniques can cultivate fewer types of bacteria, aiming to isolate and identify specific pathogens, guide antibiotic treatment and eradicate pathogens.Macrogenomics techniques can simultaneously identify both cultured and uncultured bacteria in the flora, providing the possibility for the study of symbiotic flora. With the vigorous development of SrRNA and application of 16 gene sequencing technology, the microbial ecology research of chronic sinusitis （chronic rhinosinusitis,CRS） has once again become a hot topic.And the CRS view of microbial community composition and microbial diversity were presented. This review describes the use of 16 SrRNA gene sequencing in recent years advances in technical studies CRS microbial ecology.
传统的常规细菌培养技术能够培养出的细菌种类较少，旨在分离和鉴定特定病原体、指导抗生素治疗并根除病原体。宏基因组学技术能够同时鉴定出菌群中已培养和未培养的细菌，为共生菌群的研究提供了可能性。随着16SrRNA的蓬勃发展以及16基因测序技术的应用，慢性鼻窦炎（慢性鼻-鼻窦炎，CRS）的微生物生态学研究再度成为热点话题。并且呈现了CRS微生物群落组成及微生物多样性的观点。本综述描述了近年来16SrRNA基因测序技术在CRS微生物生态学研究中的进展。
