Booth W D
AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Babraham, U.K.
J Reprod Fertil. 1987 Nov;81(2):427-31. doi: 10.1530/jrf.0.0810427.
The pheromone binding protein 'pheromaxein' which binds the pheromonal 16-androstene steroids in the saliva of the male pig (boar), was degraded and lost its binding activity in saliva incubated in air for 72 h at 21 degrees C and 37 degrees C. However, pheromaxein and its binding activity were retained in saliva incubated for 168 h at 4 degrees C. When the 3H-labelled pheromones 5 alpha-androst-16-en-3 alpha-ol (3 alpha-androstenol), 5 alpha-androst-16-en-3-one (5 alpha-androstenone) and 5 alpha-androst-16-en-3 beta-ol (3 beta-androstenol) were incubated with boar saliva for 168 h at 21 degrees C, 3 alpha-androstenol was primarily converted to 5 alpha-androstenone and 5 alpha-androstenone to 3 beta-androstenol; 3 beta-androstenol was unchanged. Evidence was obtained for microorganisms being responsible for these steroid transformations.
在雄性猪（公猪）唾液中与信息素16 - 雄烯类固醇结合的信息素结合蛋白“pheromaxein”，在21摄氏度和37摄氏度的空气中孵育72小时的唾液中会降解并丧失其结合活性。然而，在4摄氏度下孵育168小时的唾液中，pheromaxein及其结合活性得以保留。当将3H标记的信息素5α - 雄甾 - 16 - 烯 - 3α - 醇（3α - 雄烯醇）、5α - 雄甾 - 16 - 烯 - 3 - 酮（5α - 雄烯酮）和5α - 雄甾 - 16 - 烯 - 3β - 醇（3β - 雄烯醇）与公猪唾液在21摄氏度下孵育168小时时，3α - 雄烯醇主要转化为5α - 雄烯酮，5α - 雄烯酮转化为3β - 雄烯醇；3β - 雄烯醇则未发生变化。有证据表明这些类固醇转化是由微生物引起的。
