Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ourai-Kita, Izumisano-shi, Osaka 598-8531, Japan.
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
J Vet Med Sci. 2021 Sep 15;83(9):1435-1442. doi: 10.1292/jvms.21-0341. Epub 2021 Jul 22.
Properties of macrophages and lymphocytes appearing in renal fibrosis remains to be investigated. F344 rats were injected once a week with cisplatin (2 mg/kg body weight) for 8 weeks and examined at post-final injection weeks 1, 3, 6, 9, and 12. Rats developed progressive renal fibrosis at weeks 1 to 6 as fibrosis-progress phase, and subsequent amelioration at weeks 9 and 12. CD68 M1-macrophages and major histocompatibility complex (MHC) class II macrophages remarkably increased persistently, whereas CD163 M2-macrophages slightly increased. MHC class II/CD68 and MHC class II/CD163 macrophages were present, indicating that MHC class II macrophages might have both functions of M1- and M2-macrophages. In the fibrosis-progress phase, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ for M1-factors, and transforming growth factor (TGF)-β1 and IL-10 for M2-factors tended to increase; tissue injury by M1 and fibrosis by M2 might have occurred simultaneously. Lots of CD4 and CD8 T cells appeared in close relation with MHC class II macrophages, and mainly CD4 T cells formed aggregations. In the lymphocyte aggregates collected by laser microdissection, expression of IL-17A (for Th17 cells) and forkhead box P3 (FoxP3) (for Treg) significantly increased at weeks 1 and 6, respectively; presumably, Th17 cells might be involved in tissue injury, whereas Treg might be related to fibrosis amelioration. These results suggested that macrophages and T cells may contribute interrelatedly to renal fibrosis.
在肾纤维化中出现的巨噬细胞和淋巴细胞的特性仍有待研究。F344 大鼠每周接受一次顺铂（2mg/kg 体重）注射，共 8 周，并在最后一次注射后的第 1、3、6、9 和 12 周进行检查。在第 1 至 6 周的纤维化进展阶段，大鼠逐渐发生进行性肾纤维化，随后在第 9 和 12 周得到改善。CD68 M1 型巨噬细胞和主要组织相容性复合体（MHC）II 型巨噬细胞显著持续增加，而 CD163 M2 型巨噬细胞略有增加。MHC II/CD68 和 MHC II/CD163 巨噬细胞存在，表明 MHC II 型巨噬细胞可能具有 M1 和 M2 巨噬细胞的双重功能。在纤维化进展阶段，M1 因子的白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α 和干扰素（IFN）-γ，以及 M2 因子的转化生长因子（TGF）-β1 和 IL-10 倾向于增加；M1 的组织损伤和 M2 的纤维化可能同时发生。大量的 CD4 和 CD8 T 细胞与 MHC II 型巨噬细胞密切相关，并主要由 CD4 T 细胞形成聚集。在通过激光微切割收集的淋巴细胞聚集物中，IL-17A（Th17 细胞）和叉头框 P3（FoxP3）（Treg）的表达分别在第 1 和 6 周显著增加；推测 Th17 细胞可能参与组织损伤，而 Treg 可能与纤维化改善有关。这些结果表明，巨噬细胞和 T 细胞可能相互作用参与肾纤维化。
