Influence of Pyroligneous Acid on Fermentation Parameters, CO Production and Bacterial Communities of Rice Straw and Stylo Silage.

Carbon dioxide (CO) is a primary greenhouse gas and the main cause of global warming. Respiration from plant cells and microorganisms enables CO to be produced during ensiling, a method of moist forage preservation applied worldwide. However, limited information is available regarding CO emissions and mitigation during ensiling. Pyroligneous acid, a by-product of plant biomass pyrolysis, has a strong antibacterial capacity. To investigate CO production and the influence of pyroligneous acid, fresh stylo, and rice straw were ensiled with or without 1% or 2% pyroligneous acid. Dynamics of the fermentation characteristics, CO production, and bacterial communities during ensiling were analyzed. Pyroligneous acid increased the lactic acid content and decreased the weight losses, pH, ammonia-N content, butyric acid content, and coliform bacterial numbers (all < 0.05). It also increased the relative abundance of and decreased the relative abundances of harmful bacteria such as and . Adding pyrolytic acids reduced the gas production, especially of CO. It also increased the relative abundances of CO-producing bacterial genera and of genera with the potential for CO fixation. In conclusion, adding pyroligneous acid improved the fermentation quality of the two silages. During ensiling, CO production was correlated with bacterial community alterations. Using pyroligneous acid altered the bacterial community to reduce CO production during ensiling. Given the large production and demand for silage worldwide, application of pyroligneous acid may be an effective method of mitigating global warming via CO emissions.