Altering bacterial community: A possible way of lactic acid bacteria inoculants reducing CO production and nutrient loss during fermentation.

CO production during ensiling is a source of greenhouse gases emissions and a cause of nutrient loss of silage. To investigate the influence of additives on CO production and relevant bacterial communities, stylo and rice straw were ensiled with sucrose and Lactobacillus plantarum (LP). After 30 days fermentation, LP reduced CO production (from 66.2 to 0 mL/100 g fresh matter in stylo, from 83.7 to 16.6 mL/100 g fresh matter in rice straw) and weight loss (from 2.71 to 1.72% in stylo, from 2.75 to 2.40% in rice straw). CO production was positively correlated (P < 0.05) with Lactococcus (0.99), Leuconostoc (0.55), Lachnoclostridium (0.45), Prevotella (0.23) and was negatively correlated (P < 0.05) with Serratia (-0.66), Sphingobacterium (-0.58), Pediococcus (-0.36). LP decreased the relative abundance of genera positively correlated with CO production and increased that of genera negatively correlated. In conclusion, LP could reduce CO production by altering bacterial community during ensiling.