Li Min, Feng Jueping, Gao Chang, Sun Wen, Chen Yilian
Department of Oncology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430034, Hubei Province, China.
Am J Transl Res. 2021 Jun 15;13(6):6620-6628. eCollection 2021.
This research intended to explore the content and the role of miR-338 and miR-20a in the serum of patients with gastric carcinoma (GC).
Sixty-seven patients with GC, diagnosed and treated for the first time in our hospital from February 2014 to October 2016 were selected as the observation group (OG), and 45 healthy people were selected as the control group (CG). miR-338 and miR-20a of the CG and the OG were tested using qRT-PCR, and the correlation between the two indexes was analyzed by Pearson test. The diagnostic value of miR-338 and miR-20a in GC was analyzed by receiver operating characteristic curve (ROC). The correlation of miR-338 and miR-20a with clinical data was compared, and the correlation of the two with the survival of patients was observed. The independent prognostic factors in patients with GC were analyzed by Cox regression.
miR-338 expression was low in GC patients' serum, while miR-20a was high in GC patients. The expression of the two indexes was negatively correlated (r=-0.609, P<0.001). The areas under the curve of miR-338 and miR-20a were 0.849 and 0.865 respectively. Low expression of miR-338 and high expression of miR-20a were correlated to large tumors, low differentiation degree, high possibility of lymph node metastasis, and late TNM stage of GC patients. Multivariate Cox results revealed that tumor size, lymph node metastasis, differentiation degree, TNM stage, miR-338 and miR-20a were independent prognostic factors.
miR-338 and miR-20a are expected to be serological indicators for GC diagnosis and prognosis.
本研究旨在探讨微小RNA-338（miR-338）和微小RNA-20a（miR-20a）在胃癌（GC）患者血清中的含量及作用。
选取2014年2月至2016年10月在我院首次诊断并治疗的67例GC患者作为观察组（OG），选取45例健康人作为对照组（CG）。采用实时荧光定量聚合酶链反应（qRT-PCR）检测CG和OG的miR-338和miR-20a，并通过Pearson检验分析两项指标之间的相关性。通过受试者工作特征曲线（ROC）分析miR-338和miR-20a在GC中的诊断价值。比较miR-338和miR-20a与临床资料的相关性，观察两者与患者生存情况的相关性。采用Cox回归分析GC患者的独立预后因素。
GC患者血清中miR-338表达较低，而miR-20a较高。两项指标的表达呈负相关（r=-0.609，P<0.001）。miR-338和miR-20a的曲线下面积分别为0.849和0.865。miR-338低表达和miR-20a高表达与GC患者肿瘤体积大、分化程度低、淋巴结转移可能性高及TNM分期晚相关。多因素Cox结果显示，肿瘤大小、淋巴结转移、分化程度、TNM分期、miR-338和miR-20a是独立的预后因素。
miR-338和miR-20a有望成为GC诊断和预后的血清学指标。
