Akbari Hossein, Ahmadi Mehdi, Fatemi Mohammad Javad, Foroutan Ali, Akbari Peyman, Bagheri Hossein, Golkar Majid
Department of Plastic and Reconstructive Surgery, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Department of Anesthesiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
World J Plast Surg. 2021 May;10(2):76-81. doi: 10.29252/wjps.10.2.76.
Randomized skin flaps have been used as a basic treatment modality for covering skin defects for a long time but they have always been in the risk of an inherent ischemia. Fibroblast growth factor 1 is a known angiogenic factor in in vitro studies which has shown conflicting results in in vivo investigation. We aimed to determine the effect of recombinant fibroblast growth factor on the angiogenesis rate of random cutaneous flap in animal model of rats.
This experimental study was conducted on 24 adult male rats randomized to 2 groups. In the first group FGF1 was injected subdermally in equally divided doses and distances of random flap surface in days 1, 3 and 5. In second group, normal saline was injected as control. Flap surgery was done on day 21 after first injection. The extent of necrosis and angiogenesis (mean vessel density) were assessed in day 14 after surgery.
The mean percentage of clinically apparent necrosis was 35.2% (±10.5) in intervention (FGF1) group and 38.1% (±8.7) in control (normal saline), respectively. Mean vessel density was 86.20±5.6/mm2 in control group and 90.17±5.5/mm2 in intervention group, which showed no statistically significant difference.
Mean vessel density and mean percentage of clinically apparent necrosis area were similar in 2 groups of rats with random cutaneous flaps receiving FGF1 or normal saline.
随机皮瓣长期以来一直作为覆盖皮肤缺损的一种基本治疗方式，但它们始终存在内在缺血的风险。成纤维细胞生长因子1在体外研究中是一种已知的血管生成因子，然而在体内研究中其结果存在矛盾。我们旨在确定重组成纤维细胞生长因子对大鼠动物模型中随机皮瓣血管生成率的影响。
本实验研究对24只成年雄性大鼠进行，随机分为2组。第一组在第1、3和5天，将FGF1以等剂量和随机皮瓣表面等距离进行皮下注射。第二组注射生理盐水作为对照。在首次注射后第21天进行皮瓣手术。在术后第14天评估坏死程度和血管生成（平均血管密度）。
干预（FGF1）组临床明显坏死的平均百分比分别为35.2%（±10.5），对照组（生理盐水）为38.1%（±8.7）。对照组平均血管密度为86.20±5.6/mm²，干预组为90.17±5.5/mm²，差异无统计学意义。
在接受FGF1或生理盐水的两组随机皮瓣大鼠中，平均血管密度和临床明显坏死面积的平均百分比相似。
