成年小鼠脑内小胶质细胞移植（Mr MT）替代小胶质细胞。
Microglia replacement by microglia transplantation (Mr MT) in the adult mouse brain.
机构信息
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200032, China.
出版信息
STAR Protoc. 2021 Jul 9;2(3):100665. doi: 10.1016/j.xpro.2021.100665. eCollection 2021 Sep 17.
Mutations in microglia may cause brain disorders. Replacement of dysfunctional microglia by allogeneic wild-type microglia from bone marrow transplantation (Mr BMT) or peripheral blood can correct the gene deficiency at the brain-wide scale but cannot achieve precise replacement at specific brain regions. Here, we introduce a strategy with potential clinical relevance-microglia replacement by microglia transplantation (Mr MT), combining tamoxifen-induced ablation of Mr BMT cells and intracranial injection of microglia to mouse brain, to achieve region-sepcific microglia replacement. The original abbreviation of this microglia replacement strategy is mrMT. We hereby change the name to Mr MT. For complete details on the use and execution of this protocol, please refer to Xu et al. (2020).
小胶质细胞突变可能导致脑部疾病。通过骨髓移植（Mr BMT）或外周血中的同种异体野生型小胶质细胞替代功能失调的小胶质细胞，可以在全脑范围内纠正基因缺陷，但不能在特定脑区实现精确替代。在这里，我们介绍一种具有潜在临床相关性的策略——小胶质细胞移植（Mr MT）替代小胶质细胞，结合他莫昔芬诱导的 Mr BMT 细胞消融和小胶质细胞脑内注射到小鼠脑内，以实现特定脑区的小胶质细胞替代。这种小胶质细胞替代策略的原始缩写是 mrMT。我们特此将其更名为 Mr MT。有关此方案使用和执行的完整详细信息，请参阅 Xu 等人。（2020 年）。
Zotero 插件