Jalali Amir Hossein, Mozdarani Hossein, Ghanaati Hossein
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Email:
Cell J. 2021 Aug;23(3):335-340. doi: 10.22074/cellj.2021.7258. Epub 2021 Jul 17.
To evaluate the effect of contrast enhanced abdominopelvic magnetic resonance imaging (MRI), using a 3 Tesla scanner, on expression and methylation level of and genes in human peripheral blood lymphocytes.
In this prospective study, blood samples were obtained from 20 volunteer patients with mean age of 43 ± 8 years (range 32-68 years) before contrast enhanced MRI, 2 hours and 24 hours after contrast enhanced abdominopelvic 3 Tesla MRI. After separation of mononuclear cells from peripheral blood, using Ficoll-Hypaque, we analyzed gene expression changes of and genes 2 hours and 24 hours after MRI using quantitative reverse transcription polymerase chain reaction (qRT-PCR). We also evaluated methylation percentage of the above mentioned genes in before, 2 hours and 24 hours after MRI, using MethySYBR method.
Fold change analysis, in comparison with the baseline, respectively showed 1.1 ± 0.7 and 0.8 ± 0.5 mean of gene expressions in 2 and 24 hours after MRI for , while the results were 1.4 ± 0.6 and 1.4 ± 1 for (P>0.05). Methylation of the gene promoter were 8.8 ± 1.5%, 9 ± 0.6% and 9 ± 0.8% in before contrast enhanced MRI, 2 and 24 hours after contrast enhanced MRI, respectively (P>0.05). Methylation of AKT gene promoter in before contrast enhanced MRI, 2 hours and 24 hours after contrast enhanced MRI was 5.4 ± 2.5, 5 ± 3.2, 4.9 ± 2.9 respectively (P>0.05).
Contrast enhanced abdominopelvic MRI using 3 Tesla scanner apparently has no negative effect on the expression and promoter methylation level of and genes involved in the repair pathways of genome.
评估使用3特斯拉扫描仪进行的对比增强腹部盆腔磁共振成像（MRI）对人外周血淋巴细胞中 和 基因的表达及甲基化水平的影响。
在这项前瞻性研究中，从20名平均年龄为43±8岁（范围32 - 68岁）的志愿者患者身上采集血样，分别在对比增强MRI前、对比增强腹部盆腔3特斯拉MRI后2小时和24小时采集。使用Ficoll - Hypaque从外周血中分离出单核细胞后，我们采用定量逆转录聚合酶链反应（qRT - PCR）分析MRI后2小时和24小时 和 基因的表达变化。我们还使用MethySYBR方法评估MRI前、MRI后2小时和24小时上述基因的甲基化百分比。
与基线相比，倍数变化分析显示，MRI后2小时和24小时 的基因表达平均值分别为1.1±0.7和0.8±0.5，而 的结果分别为1.4±0.6和1.4±1（P>0.05）。对比增强MRI前、对比增强MRI后2小时和24小时， 基因启动子的甲基化分别为8.8±1.5%、9±0.6%和9±0.8%（P>0.05）。对比增强MRI前、对比增强MRI后2小时和24小时，AKT基因启动子的甲基化分别为5.4±2.5、5±3.2、4.9±2.9（P>0.05）。
使用3特斯拉扫描仪进行的对比增强腹部盆腔MRI显然对参与基因组修复途径的 和 基因的表达及启动子甲基化水平没有负面影响。
