Jalali Amir Hossein, Mozdarani Hossein, Ghanaati Hossein
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Eur J Radiol. 2020 Aug;129:109037. doi: 10.1016/j.ejrad.2020.109037. Epub 2020 May 4.
To evaluate the effects of contrast enhanced abdominopelvic magnetic resonance imaging (MRI) on DNA damage.
For this study, blood samples of 20 volunteers (15 women and 5 men) with mean age of 43 ± 8 years were assessed. The mean age of women was 41.4 ± 8.9 years and mean age of men was 48.5 ± 4.9 years (P = 0.14). Peripheral blood samples were collected before, 2 and 24 h after MRI in heparin and ethylenediaminetetraacetic acid (EDTA) containing tubes. Heparinized blood was cultured to assess the cytogenetic effects using cytokinesis blocked micronucleus (CBMN) assay. After isolation of mononuclear cells, alterations in genes involved in repair (CHEK2, p21) and apoptosis (BAX, BCL2) were analyzed using real-time polymerase chain reaction (qRT-PCR).
The mean number of MN in binucleated cells at before, 2 and 24 h after MRI were 17.9 ± 2.9, 18.1 ± 2.4 and 18.3 ± 2.6, respectively (p > 0.05). Results of gene expression according to fold change compared with the baseline were 1.2 ± 0.6 and 1.02 ± 0.5 at 2 and 24 h after MRI for CHEK2, and 1.3 ± 0.7 and 1.7 ± 0.7 for CDKN1A (p21); respectively (p > 0.05). Gene expression based on fold change compared with baseline were 0.9 ± 0.6 and 1.2 ± 0.8 at 2 and 24 h after MRI for BAX, and 1.05 ± 0.3 and 1.1 ± 0.7 for BCL2; respectively (p > 0.05).
Contrast enhanced abdominopelvic MRI showed no adverse effect on DNA in terms of MN formation and alterations in expression levels of some genes involved in repair and apoptosis pathways.
评估对比增强腹部盆腔磁共振成像（MRI）对DNA损伤的影响。
本研究评估了20名志愿者（15名女性和5名男性）的血样，其平均年龄为43±8岁。女性的平均年龄为41.4±8.9岁，男性的平均年龄为48.5±4.9岁（P = 0.14）。在MRI检查前、检查后2小时和24小时，采集外周血样，置于含有肝素和乙二胺四乙酸（EDTA）的试管中。对肝素化血液进行培养，使用胞质分裂阻滞微核（CBMN）试验评估细胞遗传学效应。分离单核细胞后，使用实时聚合酶链反应（qRT-PCR）分析参与修复（CHEK2、p21）和凋亡（BAX、BCL2）的基因变化。
MRI检查前、检查后2小时和24小时双核细胞中的微核平均数分别为17.9±2.9、18.1±2.4和18.3±2.6（p>0.05）。与基线相比，MRI检查后2小时和24小时CHEK2基因表达的倍数变化结果分别为1.2±0.6和1.02±0.5，CDKN1A（p21）分别为1.3±0.7和1.7±0.7（p>0.05）。与基线相比，MRI检查后2小时和24小时BAX基因表达的倍数变化分别为0.9±0.6和1.2±0.8，BCL2分别为1.05±0.3和1.1±0.7（p>0.05）。
对比增强腹部盆腔MRI在微核形成以及参与修复和凋亡途径的一些基因表达水平改变方面，对DNA没有不良影响。
