Kahalerras Labiba, Otmani Ines, Abdennour Cherif
Laboratory of Animal Ecophysiology, Department of Biology, Faculty of Sciences, University of Badji Mokhtar-Annaba, 23000, Annaba, Algeria.
Biol Trace Elem Res. 2022 May;200(5):2205-2222. doi: 10.1007/s12011-021-02818-8. Epub 2021 Jul 26.
The current study investigates the potential alleviating activity of bulbs (B) and leaves (L) of Allium triquetrum aqueous extract (ATE) on repro-toxicity induced by lead acetate (Pb) in male Wistar rats administrated orally for 3 consecutive weeks. Eighteen groups of rats were divided into the control, Pb (500 mg/kg body weight/day), positive controls of B and L (2 g, 3 g, 4 g, 6 g/kg body weight/day), in addition to four mixtures of each of Pb-B (Pb-B1, Pb-B2, Pb-B3, Pb-B4) and Pb-L (Pb-L1, Pb-L2, Pb-L3, Pb-L4). The two extracts were subjected to phytochemical screening and HPLC analysis. Sperm characteristics were evaluated by CASA system, as well as the serum testosterone, testicular and epididymal levels of glutathione (GSH), glutathione peroxidase (GPx), and malondialdehyde (MDA). The phytochemical screening proved that bulbs' and leaves' extracts were rich in various compounds and the HPLC showed that leaves contain more tannins. Results revealed a significant decrease in the testicular and in the epididymal weights, sperm concentration, motility, testosterone, velocity, vitality, round cells, GSH, and GPx levels in the Pb-intoxicated rats compared to the control, with the exception of MDA concentration that was significantly increased. However, the co-administration of garlic extracts (Pb-B and Pb-L) exhibited a significant increase in all mentioned markers, except for the MDA level which was reduced. Likewise, Pb caused histological injuries in the testicular seminiferous of rats, while the co-administration of wild garlic has reduced such effect, especially in the higher doses. Both extracts of Pb-B and Pb-L have attenuated Pb toxicity in a dose-dependent manner. In conclusion, aqueous extracts of A. triquetrum have the potential to reduce Pb testicular injuries by boosting sperm characteristics and ameliorating oxidative stress markers.
本研究调查了三棱葱水提取物（ATE）的鳞茎（B）和叶片（L）对连续3周口服醋酸铅（Pb）诱导的雄性Wistar大鼠生殖毒性的潜在缓解作用。将18组大鼠分为对照组、Pb组（500 mg/kg体重/天）、B和L的阳性对照组（2 g、3 g、4 g、6 g/kg体重/天），此外还有Pb-B（Pb-B1、Pb-B2、Pb-B3、Pb-B4）和Pb-L（Pb-L1、Pb-L2、Pb-L3、Pb-L4）各四种混合物组。对两种提取物进行了植物化学筛选和高效液相色谱分析。通过计算机辅助精子分析系统评估精子特征，以及血清睾酮、睾丸和附睾中的谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）和丙二醛（MDA）水平。植物化学筛选证明鳞茎和叶片提取物富含多种化合物，高效液相色谱显示叶片含有更多单宁。结果显示，与对照组相比，Pb中毒大鼠的睾丸和附睾重量、精子浓度、活力、睾酮、速度、活力、圆形细胞、GSH和GPx水平显著降低，但MDA浓度显著升高。然而，大蒜提取物（Pb-B和Pb-L）联合给药后，除MDA水平降低外，所有上述指标均显著升高。同样，Pb对大鼠睾丸生精组织造成了组织学损伤，而野生大蒜联合给药减轻了这种影响，尤其是在高剂量时。Pb-B和Pb-L两种提取物均以剂量依赖性方式减轻了Pb毒性。总之，三棱葱水提取物有潜力通过改善精子特征和减轻氧化应激指标来减少Pb对睾丸的损伤。
