Laboratory of Animal Ecophysiology, Department of Biology, Faculty of Sciences, University Badji Mokhtar-Annaba, 23000, Annaba, Algeria.
Biol Trace Elem Res. 2022 Nov;200(11):4733-4743. doi: 10.1007/s12011-021-03052-y. Epub 2022 Jan 2.
The aim of this study was to scrutinize the possible mitigating role of leaves' Allium triquetrum L. against the toxicity of lead acetate on liver and kidney markers of Wistar rat. Lead acetate (Pb) and leaves' aqueous extracts (L) were orally administrated for 3 weeks. Rats were divided into the control, Pb group (500 mg/kg body weight/day), positive controls L (2g, 3g, 4g/kg BW/day), along with three combined groups of the same doses (Pb-L1, Pb-L2, Pb-L3). The levels of plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total proteins (TP), albumin (ALB), urea, creatinine (Cr), and uric acid (UA), as well as the hepatic and the renal malondialdehyde (MDA), glutathione (GSH), and glutathione peroxidase (GPx), were estimated. Results exhibited a significant increase in plasma AST, ALT, ALP, urea, creatinine, uric acid, and MDA levels of the Pb group compared to the control, with the exception of TP, ALB, GSH levels, and GPx activities that were significantly diminished, though the co-administration of garlic extracts (Pb-L) revealed a significant decrease in all mentioned markers, excluding the TP, ALB, GSH, and GPx levels. Likewise, Pb caused histological injuries in the hepatic and renal tissues of rats, while the co-administration of leaves' wild garlic has reduced such effect. Thought, the Pb-L has attenuated the Pb-induced toxicity in a dose-dependent manner. In conclusion, the aqueous extracts of A. triquetrum have the potential to alleviate Pb hepatotoxicity and nephrotoxicity through the modulation of most biomarkers in Wistar rat.
本研究旨在探讨三楞草（Allium triquetrum L.）叶片对醋酸铅致 Wistar 大鼠肝、肾功能损伤标志物的可能缓解作用。醋酸铅（Pb）和叶片水提物（L）经口给药 3 周。将大鼠分为对照组、Pb 组（500mg/kg 体重/天）、阳性对照 L 组（2g、3g、4g/kg BW/天），以及相同剂量的三个联合组（Pb-L1、Pb-L2、Pb-L3）。测定血浆天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、总蛋白（TP）、白蛋白（ALB）、尿素、肌酐（Cr）和尿酸（UA）水平，以及肝、肾丙二醛（MDA）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）水平。结果显示，与对照组相比，Pb 组血浆 AST、ALT、ALP、尿素、肌酐、尿酸和 MDA 水平显著升高，除 TP、ALB、GSH 水平和 GPx 活性显著降低外，大蒜提取物（Pb-L）联合给药显著降低了所有上述标志物的水平，除 TP、ALB、GSH 和 GPx 水平外。同样，Pb 导致大鼠肝、肾组织的组织学损伤，而野生三楞草叶片水提物的联合给药减轻了这种作用。虽然，Pb-L 以剂量依赖的方式减轻了 Pb 诱导的毒性。总之，三楞草叶片水提物通过调节 Wistar 大鼠的大多数生物标志物，具有缓解 Pb 肝毒性和肾毒性的潜力。
