Impact of the PPG Sampling Rate in the Pulse Rate Variability Indices Evaluating Several Fiducial Points in Different Pulse Waveforms.

The main aim of this work is to study the effect of the sampling rate of the photoplethysmographic (PPG) signal for pulse rate variability (PRV) analysis. Forehead and finger PPG signals were recorded at 1000 Hz during a rest state, with red and infrared wavelengths, simultaneously with the electrocardiogram (ECG). The PPG sampling rate has been reduced by decimation, obtaining signals at 500 Hz, 250 Hz, 125 Hz, 100 Hz, 50 Hz and 25 Hz. Five fiducial points were computed: apex, up-slope, medium, line-medium and medium interpolate point. The medium point is located in the middle of the up-slope of the pulse. The medium interpolate point is a new proposal as fiducial point that consider the abrupt up-slope of the PPG pulse, so it can be recovered by linear interpolation when the sampling rate is reduced. The error performed in the temporal location of the fiducial points was computed. Pulse period time interval series were obtained from all PPG signals and fiducial points, and compared with the RR intervals obtained from the ECG. Heart rate variability and PRV signals were estimated and classical time and frequency domain indices were computed. The results showed that the medium interpolate point of the PPG pulse was the most accurate fiducial point under different PPG morphologies and sensor locations, when sampling rate was reduced. Being able to reduce the sampling rate to 50 Hz without causing significant changes in time and frequency indices, when medium interpolate point was used as fiducial point.