First Report of a Multidrug-Resistant ST58 Harboring Extended-Spectrum Beta-Lactamase of the CTX-M-1 Class in a Fecal Sample of a Captive Baird's Tapir () in Costa Rica, Central America.

This study's main objective was to analyze the antibiotic susceptibility profile of isolates obtained from a fecal sample of a captive Baird's tapir () in Costa Rica. The fecal sample was collected inside the enclosure on March 3, 2017, right after the animal defecated. Samples were cultured on MacConkey agar plates nonsupplemented and supplemented with 2 μg/mL of cefotaxime. Bacterial identification and antibiotic susceptibility were performed with the Vitek 2 Compact System and the Kirby Bauer disk diffusion method, respectively. Polymerase chain reaction amplification was performed to detect beta-lactamase genes. Resistant isolates were subjected to whole-genome sequencing (WGS). After evaluating several antibiotic classes, a multidrug-resistant strain with extended-spectrum beta-lactamase phenotype was isolated. Resistance to cefotaxime, cefepime, ampicillin, ampicillin/sulbactam, and tetracycline was detected. WGS analysis showed the presence of , , and (B) genes. The presence of IncN plasmids and Col156 was also detected. Our findings are according with the notion that animals' high density enhances the spread of resistant determinants in a captive environment in a limited space, where the likelihood of direct or indirect contact with other animals and humans is more frequent.