Departments of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Departments of Systems Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Biotechnol Appl Biochem. 2022 Aug;69(4):1576-1586. doi: 10.1002/bab.2229. Epub 2021 Aug 7.
The high incidence rate of CRC demands early diagnosis of the disease and readiness of diagnostic biomarker. In present study, we have investigated c-MYC, AXIN1, and COL11A1 expression levels in course of CRC progression and their correlation with demographics and clinical risk factors. Fifty-five tumors and 41 normal tissues were obtained from Tumor Bank of Iran, total RNA was extracted, cDNA was synthesized, and RT-qPCR was performed. Results were analyzed using Rest 2009 and SPSS software. Analysis at mRNA level showed upregulation of the two genes; c-MYC with a p-value of 0.001 and COL11A1 with an observed p-value of 0.02, while a p-value of 0.04 indicated AXIN1 downregulation. The observed overexpression of COL11A1 in stage 0 compared to other stages of CRC asserts importance of this gene in CRC prognosis. Moreover, statistical analysis confirms a significant correlation between expression of these genes and several clinical risk factors of CRC. Our study supports the importance of the studied genes and provides further information regarding the molecular mechanism of CRC. Further studies on these genes could elucidate their pivotal role for both early detection and/or diagnosis of CRC in addition to have important biomarkers for CRC management available.
CRC 的高发病率要求对疾病进行早期诊断，并准备好诊断生物标志物。在本研究中，我们研究了 CRC 进展过程中 c-MYC、AXIN1 和 COL11A1 的表达水平及其与人口统计学和临床危险因素的相关性。从伊朗肿瘤库获得了 55 个肿瘤和 41 个正常组织，提取总 RNA，合成 cDNA，并进行 RT-qPCR。使用 Rest 2009 和 SPSS 软件分析结果。mRNA 水平的分析表明这两个基因上调；c-MYC 的 p 值为 0.001，COL11A1 的观察到的 p 值为 0.02，而 AXIN1 的下调 p 值为 0.04。与 CRC 的其他阶段相比，COL11A1 在 0 期的过度表达表明该基因在 CRC 预后中的重要性。此外，统计学分析证实了这些基因的表达与 CRC 的几个临床危险因素之间存在显著相关性。我们的研究支持了所研究基因的重要性，并提供了有关 CRC 分子机制的进一步信息。对这些基因的进一步研究可以阐明它们在 CRC 的早期检测和/或诊断中的关键作用，以及为 CRC 管理提供重要的生物标志物。
