Betterle C, Peserico A, Bersani G, Rigon F, Del Prete G
Ric Clin Lab. 1977 Jul-Sep;7(3):242-51.
Antibodies to epidermal cytoplasmic antigens were detected by the indirect immunofluorescence (IF) technique in 36% of 100 adult healthy subjects and in 17.6% of 17 normal newborn infants. This type of autoantibody occurred in 33% of 100 cases with vitiligo, in 32.5% of 40 cases with psoriasis, in 55.3% of patients with malignant tumours and in 72.7% of subjects with malignant melanoma. The frequency of the autoimmune reactions was statistically significant only in patients with malignant neoplasms. In the majority of positive cases the IF pattern involved the upper layers of the epidermal cells (U-CYT). The basal layer was generally negative. Only a few cases showed a pattern involving both the upper and the basal layers (G-CYT). However, a wide variation in staining was noted when sera were tested on different skin specimens or different sections of the same skin. To identify the nature of the target antigen(s), absorption experiments of sera were attempted with lyophilized and particulate antigens. Animal and human blood cells and lyophilized homogenates of malignant tumours failed to absorb the autoimmune activity of positive sera. Only a powder preparation of keratin induced a decrease in antibody titres. It is postulated that they are the result of an antigenic stimulation by exogenous substances commonly present in the environment.
采用间接免疫荧光（IF）技术，在100名健康成人中有36%检测到针对表皮细胞质抗原的抗体，在17名正常新生儿中有17.6%检测到该抗体。在100例白癜风患者中有33%出现这种自身抗体，在40例银屑病患者中有32.5%出现，在恶性肿瘤患者中有55.3%出现，在恶性黑色素瘤患者中有72.7%出现。自身免疫反应的频率仅在恶性肿瘤患者中具有统计学意义。在大多数阳性病例中，IF模式涉及表皮细胞的上层（U-CYT）。基底层通常为阴性。只有少数病例显示出同时涉及上层和基底层的模式（G-CYT）。然而，当用不同的皮肤标本或同一皮肤的不同切片检测血清时，发现染色存在很大差异。为了确定靶抗原的性质，尝试用冻干抗原和颗粒抗原对血清进行吸收实验。动物和人类血细胞以及恶性肿瘤的冻干匀浆均未能吸收阳性血清的自身免疫活性。只有角蛋白粉制剂能使抗体滴度降低。据推测，它们是由环境中普遍存在的外源性物质引起的抗原刺激的结果。
