原儿茶醛通过调节 NF-B 和 MAPK 活性抑制 RAW264.7 和 BMM 细胞的破骨细胞分化。

Protocatechualdehyde Inhibits the Osteoclast Differentiation of RAW264.7 and BMM Cells by Regulating NF-B and MAPK Activity.

机构信息

Department of Central Lab, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.

The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.

出版信息

Biomed Res Int. 2021 Jul 16;2021:6108999. doi: 10.1155/2021/6108999. eCollection 2021.

Protocatechualdehyde (PCA), an important component of , has many activities, such as anti-inflammatory and antisepsis activities. However, the role of PCA in osteoclasts is not clear. We used RAW264.7 cells (a mouse leukemic monocyte/macrophage cell line) and bone marrow macrophages (BMMs) to probe the role of PCA in osteoclasts and the underlying mechanism. The effects of PCA on cell activity were evaluated with CCK-8 assays. TRAP staining detected mature osteoclasts. Corning Osteo Assay Surface plates were used to examine absorption. The levels of RNA and protein were analyzed, respectively, using RT-PCR and Western blotting. PCA (5 g/ml) was not toxic to the two cell types but reduced the formation of osteoclasts and bone absorption. Furthermore, PCA restrained the expression of mRNAs encoding proteins associated with osteoclasts and reduced the phosphorylation of proteins in important signaling pathways. The results indicate that PCA inhibits osteoclast differentiation by suppressing NF-B and MAPK activity.

原儿茶醛（PCA）是的重要组成部分，具有多种活性，如抗炎和抗菌活性。然而，PCA 在破骨细胞中的作用尚不清楚。我们使用 RAW264.7 细胞（一种小鼠白血病单核/巨噬细胞系）和骨髓巨噬细胞（BMMs）来探讨 PCA 在破骨细胞中的作用及其潜在机制。用 CCK-8 法评估 PCA 对细胞活性的影响。TRAP 染色检测成熟破骨细胞。使用康宁 Osteo Assay Surface 板检测吸收。分别使用 RT-PCR 和 Western blot 分析 RNA 和蛋白质的水平。5μg/ml 的 PCA 对这两种细胞类型没有毒性，但减少了破骨细胞的形成和骨吸收。此外，PCA 抑制与破骨细胞相关的蛋白编码 mRNAs 的表达，并降低重要信号通路中蛋白的磷酸化。结果表明，PCA 通过抑制 NF-B 和 MAPK 活性抑制破骨细胞分化。