聚乙烯醇/三聚偏磷酸钠作为兔实验性玻璃体切除模型中玻璃体替代物的生物相容性
Biocompatibility of polyvinyl alcohol/trisodium trimetaphosphate as vitreous substitute in experimental vitrectomy model in rabbits.
作者信息
de Oliveira Ramon Antunes, Muralha Felipe Picanço, Grupenmacher Alex Treiger, de Araújo Morandim-Giannetti Andreia, Bersanetti Patrícia Alessandra, Maia Maurício, Magalhães Junior Octaviano
机构信息
Department of Ophthalmology and Visual Sciences, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
Department of Chemical Engineering, Centro Universitário FEI, São Paulo, Brazil.
出版信息
J Biomed Mater Res B Appl Biomater. 2022 Feb;110(2):460-466. doi: 10.1002/jbm.b.34923. Epub 2021 Jul 29.
Synthetic hydrogels have been proposed as vitreous substitutes recently. This study aims to evaluate the biocompatibility of polyvinyl alcohol (PVA) crosslinked with trisodium trimetaphosphate (SMTP) hydrogel in rabbit vitrectomized eyes. Seven animals were submitted to pars plana vitrectomy and the vitreous was replaced by PVA/SMTP hydrogel. Optical coherence tomography, fluorescein angiogram, clinical, and electrophysiological (ERG) examinations were analyzed at baseline, on postoperative days 7 and 30. The fellow eye was used as the control group. Hydrogel opacification was observed and ERG recordings were reduced in the hydrogel group in rod response, b-wave cone response and flicker. A histological analysis showed retinal disorganization, presence of multinucleated cells, and intraretinal hydrogel particles. The PVA/SMTP hydrogel showed poor biocompatibility. Novel biomaterials compounds should be analyzed in vivo.
近年来，合成水凝胶已被提议作为玻璃体替代物。本研究旨在评估用三聚磷酸钠（SMTP）交联的聚乙烯醇（PVA）水凝胶在兔玻璃体切除眼中的生物相容性。七只动物接受了扁平部玻璃体切除术，玻璃体被PVA/SMTP水凝胶替代。在基线、术后第7天和第30天对光学相干断层扫描、荧光素血管造影、临床和电生理（ERG）检查进行分析。对侧眼用作对照组。在水凝胶组中观察到水凝胶混浊，并且在视杆细胞反应、b波视锥细胞反应和闪烁反应中ERG记录减少。组织学分析显示视网膜结构紊乱、多核细胞的存在以及视网膜内水凝胶颗粒。PVA/SMTP水凝胶显示出较差的生物相容性。新型生物材料化合物应在体内进行分析。
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