Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung, 81157, Taiwan.
Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 81157, Taiwan.
Environ Pollut. 2021 Nov 1;288:117800. doi: 10.1016/j.envpol.2021.117800. Epub 2021 Jul 17.
There has been a significant increase in the microplastic (MP) polluting the ocean in recent time which is regarded as toxic for living organisms. In this study, Fluorescent red polyethylene microspheres (FRPE) were administered intramuscularly to Litopenaeus vannamei juveniles at the concentration of 0.1, 0.2, 0.5 and 1.0 μg (g shrimp), and the survival rate was recorded. Analysis of the hepatopancreas for antioxidant enzyme activity and gene expression were done after seven days. Further tissue morphology and accumulation of FRPE was analysed. The results showed that FRPE at 0.5 and 1.0 μg (g shrimp) reduce the survival rate of L. vannamei. FRPE at 0.5 and 1.0 μg (g shrimp) reduced superoxide dismutase (SOD) activity; FRPE at different concentrations reduced catalase (CAT) activity; FRPE at 0.2, 0.5 and 1.0 μg (g shrimp) increased the lipid peroxide thiobarbituric acid (TBARS) content. FRPE at 0.1, 0.2, and 0.5 μg (g shrimp) significantly affect the performance of SOD and CAT genes; FRPE at 0.2 and 0.5 μg (g shrimp) significantly improves GPx gene performance; FRPE at 1.0 μg (g shrimp) significantly reduced the expression of GPx genes. Analysis of tissue morphology shows that FRPE cause muscle, midgut gland, and hepatopancreas, and gill damage at different concentrations. In the results of accumulation of microplastic, FRPE accumulated in gill tissue at 0.2 and 0.5 μg (g shrimp); FRPE accumulated in gill, muscle and hepatopancreas tissue at 1.0 μg (g shrimp). Based on the above results, FRPE at 0.5 and 1.0 μg (g shrimp) can regulate the antioxidant enzymes of L. vannamei, increase lipid peroxide content, cause tissue damage by accumulating in the tissues. The rate of survival decreased in L. vannamei, and the impact of FRPE at 1.0 μg (g shrimp) was significant.
最近，海洋中的微塑料（MP）污染显著增加，被认为对生物有毒性。在这项研究中，荧光红色聚乙烯微球（FRPE）以 0.1、0.2、0.5 和 1.0μg（虾重）的浓度肌肉内给药给南美白对虾幼虾，并记录存活率。七天后分析肝胰腺的抗氧化酶活性和基因表达。进一步分析组织形态和 FRPE 的积累。结果表明，0.5 和 1.0μg（虾重）的 FRPE 降低了南美白对虾的存活率。0.5 和 1.0μg（虾重）的 FRPE 降低了超氧化物歧化酶（SOD）活性；不同浓度的 FRPE 降低了过氧化氢酶（CAT）活性；0.2、0.5 和 1.0μg（虾重）的 FRPE 增加了脂质过氧化物硫代巴比妥酸（TBARS）含量。0.1、0.2 和 0.5μg（虾重）的 FRPE 显著影响 SOD 和 CAT 基因的表达；0.2 和 0.5μg（虾重）的 FRPE 显著提高 GPx 基因的表达；1.0μg（虾重）的 FRPE 显著降低 GPx 基因的表达。组织形态学分析表明，FRPE 在不同浓度下导致肌肉、中肠腺和肝胰腺以及鳃损伤。在微塑料积累的结果中，0.2 和 0.5μg（虾重）的 FRPE 积累在鳃组织中；1.0μg（虾重）的 FRPE 积累在鳃、肌肉和肝胰腺组织中。基于上述结果，0.5 和 1.0μg（虾重）的 FRPE 可以调节南美白对虾的抗氧化酶，增加脂质过氧化物含量，通过在组织中积累造成组织损伤。南美白对虾的存活率下降，1.0μg（虾重）的 FRPE 影响显著。
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