Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan.
Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan.
Mar Pollut Bull. 2024 Aug;205:116683. doi: 10.1016/j.marpolbul.2024.116683. Epub 2024 Jul 6.
This study examines the combined effects of polyethylene microplastics (PE-MP) and copper (Cu) on the immune and oxidative response of Litopenaeus vannamei. PE-MP adsorbed with Cu at 2.3, 6.8, and 16.8 ng (g shrimp)) were injected into L. vannamei. Over 14 days, survival rates were monitored, and immune and oxidative stress parameters were assessed. The results showed that combined exposure to PE-MP and Cu significantly reduced the survival rate and decreased total haemocyte count. Immune-related parameters (phagocytic rate, phenoloxidase and superoxide dismutase (SOD)) and antioxidant-related parameters (SOD, catalase and glutathione peroxidase mRNA and enzyme) also decreased, while respiratory burst activity significantly increased, indicating immune and antioxidant system disruption. Additionally, there was a significant increase in oxidative stress, as measured by malondialdehyde levels. Histopathological analysis revealed severe muscle, hepatopancreas, and gill damage. These results suggest that simultaneous exposure to PE-MP and Cu poses greater health risks to white shrimp.
本研究考察了聚乙烯微塑料（PE-MP）和铜（Cu）对凡纳滨对虾免疫和氧化应激反应的联合影响。PE-MP 吸附 Cu 的浓度分别为 2.3、6.8 和 16.8ng（g 虾），然后将其注入凡纳滨对虾体内。在 14 天内监测存活率，并评估免疫和氧化应激参数。结果表明，PE-MP 和 Cu 的联合暴露显著降低了存活率和总血细胞计数。免疫相关参数（吞噬率、酚氧化酶和超氧化物歧化酶（SOD））和抗氧化相关参数（SOD、过氧化氢酶和谷胱甘肽过氧化物酶 mRNA 和酶）也降低，而呼吸爆发活性显著增加，表明免疫和抗氧化系统受到破坏。此外，丙二醛水平的升高表明氧化应激显著增加。组织病理学分析显示肌肉、肝胰腺和鳃严重受损。这些结果表明，PE-MP 和 Cu 的同时暴露对南美白对虾构成更大的健康风险。
