Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA.
Department of Medical Physiology, College of Medicine, Texas A&M University of Health Science Center, Bryan, TX, USA.
Methods Mol Biol. 2021;2319:25-30. doi: 10.1007/978-1-0716-1480-8_3.
Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature. RNA-sequencing (RNA-Seq), an emerging next-generation sequencing (NGS) technique, facilitates us to quantify the global gene expression levels and, thereby, provides us with a powerful tool to study the transcriptional reprograming in the cells. Here, we describe the RNA-Seq procedures and the subsequent bioinformatic analysis to understand the transcriptome regulation by FSS in lymphatic endothelial cells (LECs).
流体切应力（FSS）是一种重要的机械力，调节血液和淋巴脉管系统中的内皮和血管功能。RNA 测序（RNA-Seq）是一种新兴的下一代测序（NGS）技术，使我们能够定量测量全局基因表达水平，并为我们提供了研究细胞中转录重编程的强大工具。在这里，我们描述了 RNA-Seq 程序和随后的生物信息学分析，以了解 FSS 对淋巴管内皮细胞（LECs）中转录组的调节。
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