Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA.
Methods Mol Biol. 2021;2319:45-49. doi: 10.1007/978-1-0716-1480-8_5.
Histamine is well characterized to cause hyperpermeability in both blood and lymphatic endothelial cells (BECs and LECs) in infection and inflammation. The increased permeability impairs the barrier function of vessels to fluid, soluble electrolytes, and proteins, resulting in the swelling of interstitial tissues, termed edema and lymphedema. Here, we describe two approaches to study the permeability of LECs, to macromolecules or to electrolytes, upon histamine stimulation in vitro.
组胺在感染和炎症中很好地被描述为引起血液和淋巴管内皮细胞（BEC 和 LEC）的高通透性。通透性的增加损害了血管对液体、可溶性电解质和蛋白质的屏障功能，导致间质组织肿胀，称为水肿和淋巴水肿。在这里，我们描述了两种方法来研究 LEC 对大分子或电解质的通透性，即在体外受到组胺刺激时。
