Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
Methods Mol Biol. 2021;2319:69-75. doi: 10.1007/978-1-0716-1480-8_8.
There is increasing interest in the study of the mammalian lymphatic system, including the lymphatic endothelial cells (LECs) that make up lymphatic vessels. The ability to isolate primary LECs from tissue of normal and genetically modified mice permits detailed analysis of this unique cell type. Here, we describe a robust protocol for the isolation and in vitro expansion of LECs from mouse lung by antibody-based magnetic separation.
人们对哺乳动物淋巴系统的研究兴趣日益增加，其中包括构成淋巴管的淋巴内皮细胞（LEC）。能够从正常和基因修饰小鼠的组织中分离原代 LEC，使得对这种独特细胞类型的详细分析成为可能。在这里，我们描述了一种基于抗体的磁性分离法从小鼠肺中分离和体外扩增 LEC 的可靠方案。
