Nanomaterials for BioImaging Group, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain.
Nanomaterials for BioImaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain.
Methods Mol Biol. 2021;2350:239-251. doi: 10.1007/978-1-0716-1593-5_15.
Lifetime multiplexed imaging refers to the simultaneous labeling of different structures with fluorescent probes that present identical photoluminescence spectra and distinct fluorescence lifetimes. This technique allows extracting quantitative information from multichannel in vivo fluorescence imaging. In vivo lifetime multiplexed imaging requires fluorophores with excitation and emission bands in the near-infrared (NIR) and tunable fluorescence lifetimes, plus an imaging system capable of time-resolved image acquisition and analysis.
终身复用成像指的是同时用荧光探针标记不同的结构，这些探针具有相同的光致发光光谱和不同的荧光寿命。该技术允许从多通道活体荧光成像中提取定量信息。体内寿命复用成像需要具有近红外（NIR）激发和发射带以及可调谐荧光寿命的荧光团，以及能够进行时间分辨图像采集和分析的成像系统。
