Laboratoire de Recherche en Nanosciences LRN EA4682 and NanoMat' platform, Université de Reims Champagne-Ardenne, Reims, France.
CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne (URCA), Laboratoire SiRMa - Campus Moulin de la Housse, Reims cedex, France.
Methods Mol Biol. 2021;2350:289-297. doi: 10.1007/978-1-0716-1593-5_18.
Atomic force microscopy (AFM) enables the characterization of a wide range of samples including live cells. It is generally admitted that cancer cells are significantly softer than their normal counterparts, but imaging live cells by AFM using traditional modes can be at the cost of time or resolution. We describe how this tool can be used to estimate the motility of cancer versus normal cells, based on topographical and mechanical approaches, and coupled to optical imaging.
原子力显微镜（AFM）能够对包括活细胞在内的各种样本进行特性分析。人们普遍认为癌细胞比正常细胞软得多，但使用传统模式的 AFM 对活细胞进行成像可能会牺牲时间或分辨率。我们描述了如何基于形貌学和力学方法，结合光学成像，使用这种工具来估计癌细胞与正常细胞的运动性。
