一株新型异养硝化-好氧反硝化酵母 Barnettozyma californica K1 的特性。
Characteristics of a novel heterotrophic nitrification-aerobic denitrification yeast, Barnettozyma californica K1.
机构信息
School of Life Sciences, South China Normal University, Guangzhou 510631, PR China; Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education, Guangzhou 510631, PR China; Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou 510631, PR China.
School of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
出版信息
Bioresour Technol. 2021 Nov;339:125665. doi: 10.1016/j.biortech.2021.125665. Epub 2021 Jul 26.
Yeast strain K1, isolated from surface sediment, was identified as Barnettozyma californica. The strain showed efficient heterotrophic nitrification and aerobic denitrification (HN-AD) at initial ammonium, nitrite and nitrate concentrations of 14-140 mg/L. Additionally, the optimum carbon source for its growth and nitrogen removal activity was sucrose, followed by glucose, acetate and citrate. The maximum removal efficiencies of ammonium, nitrite and nitrate were 99.11%, 99.13% and 98.84% under 48 h of culture with sucrose at 140 mg/L nitrogen and the corresponding removal efficiencies of total nitrogen were 90.16%, 86.65% and 81.48%, respectively. The optimum conditions for the inorganic nitrogen removal and growth of strain K1 were a C/N of 18 and a salinity of 5-15 ppt. The amoA, nirK and nosZ genes of K1 were detected. All the results suggest that B. californica K1 is capable of HN-AD and has the potential to remove inorganic nitrogen from wastewater.
从表层沉积物中分离到的酵母菌株 K1 被鉴定为加利福尼亚Barnettozyma。该菌株在初始氨氮、亚硝酸盐氮和硝酸盐氮浓度分别为 14-140mg/L 时表现出高效的异养硝化和好氧反硝化（HN-AD）作用。此外，其生长和脱氮活性的最佳碳源是蔗糖，其次是葡萄糖、乙酸盐和柠檬酸盐。在 48 小时的培养过程中，当 140mg/L 氮源为蔗糖时，铵、亚硝酸盐和硝酸盐的最大去除效率分别为 99.11%、99.13%和 98.84%，相应的总氮去除效率分别为 90.16%、86.65%和 81.48%。K1 菌株无机氮去除和生长的最佳条件为 C/N 比为 18，盐度为 5-15 ppt。检测到 K1 的 amoA、nirK 和 nosZ 基因。所有结果表明，加利福尼亚 Barnettozyma K1 能够进行 HN-AD，并有潜力从废水中去除无机氮。
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