Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226019, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226019, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Poult Sci. 2021 Sep;100(9):101370. doi: 10.1016/j.psj.2021.101370. Epub 2021 Jun 30.
The extended-spectrum cephalosporin resistant E. coli from food animals transferring to community settings of humans causes a serious threat to public health. Unlike phylogroup B2 E. coli strains, the clinical significance of isolates in phylogroup F is not well revealed. Here, we report on a collection (n = 563) of phylogroup F E. coli isolates recovered from chicken colibacillosis tissues and retail raw chicken meat samples in Eastern China. There was an overlapped distribution of MLST types between chicken colibacillosis-origin and meat-source phylogroup F E. coli, including dominant STs (ST648, ST405, ST457, ST393, ST1158, etc). This study further investigated the presence of extended-spectrum β-lactamase (ESBL/pAmpC) producers in these chicken-source phylogroup F E. coli strains. The prevalence of extended-spectrum cephalosporin resistant strains in phylogroup F E. coli from chicken colibacillosis and raw meat separately accounted for 66.1 and 71.2%. The resistance genotypes and plasmid replicon types of chicken-source phylogroup F E. coli isolates were characterized by multiplex PCR. Our results revealed β-lactamase CTX-M, OXA, CMY and TEM genes were widespread in chicken-source phylogroup F E. coli, and blaCTX-M was the most predominant ESBL gene. Moreover, there was a high prevalence of non-lactamase resistance genes in these β-lactam-resistant isolates. The replicons IncB/O/K/Z, IncI1, IncN, IncFIC, IncQ1, IncX4, IncY, and p0111, associated with antibiotic-resistant large plasmids, were widespread in chicken-source phylogroup F E. coli. There was no obvious difference for the populations, resistance spectrums, and resistance genotypes between phylogroup F E. coli from chicken colibacillosis tissues and retail meats. This detail assessment of the population and resistance genotype showed chicken-source phylogroup F E. coli might hold zoonotic risk and contribute the spread of multidrug-resistant E. coli to humans.
食源性动物中产超广谱头孢菌素耐药的大肠杆菌转移到人类社区环境对公共卫生造成严重威胁。与 phylogroup B2 大肠杆菌菌株不同，phylogroup F 分离株的临床意义尚未得到充分揭示。在这里，我们报告了一组（n=563）从华东地区鸡大肠杆菌病组织和零售生鸡肉中分离的 phylogroup F 大肠杆菌。鸡大肠杆菌病起源和肉源 phylogroup F 大肠杆菌的 MLST 型存在重叠分布，包括优势 STs（ST648、ST405、ST457、ST393、ST1158 等）。本研究进一步调查了这些鸡源 phylogroup F 大肠杆菌菌株中是否存在产超广谱β-内酰胺酶（ESBL/pAmpC）的产酶菌。phylogroup F 大肠杆菌对头孢菌素的耐药率在鸡大肠杆菌病和生肉中分别为 66.1%和 71.2%。通过多重 PCR 对鸡源 phylogroup F 大肠杆菌分离株的耐药基因型和质粒复制子类型进行了表征。研究结果表明，CTX-M、OXA、CMY 和 TEM 基因在鸡源 phylogroup F 大肠杆菌中广泛存在，blaCTX-M 是最主要的 ESBL 基因。此外，这些β-内酰胺耐药分离株中还存在高比例的非β-内酰胺耐药基因。IncB/O/K/Z、IncI1、IncN、IncFIC、IncQ1、IncX4、IncY 和 p0111 等与抗生素耐药大质粒相关的复制子在鸡源 phylogroup F 大肠杆菌中广泛存在。鸡大肠杆菌病组织和零售鸡肉中 phylogroup F 大肠杆菌的种群、耐药谱和耐药基因型无明显差异。对种群和耐药基因型的详细评估表明，鸡源 phylogroup F 大肠杆菌可能具有人畜共患病风险，并有助于将多药耐药大肠杆菌传播给人类。
