一种基于化学发光的检测方法，用于检测儿童牛奶蛋白特异性 IgE，该方法具有优异的抗特异性 IgG 干扰能力。
A light-initiated chemiluminescent assay for the detection of children's milk protein-specific IgE with excellent ability to avoid interference of specific IgG.
机构信息
School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
Department of Medical Laboratory, Tianjin Children's Hospital, Tianjin, China.
出版信息
J Immunol Methods. 2021 Oct;497:113110. doi: 10.1016/j.jim.2021.113110. Epub 2021 Jul 30.
The detection of allergen-specific IgE is of value for the diagnosis of children''s milk allergy. However, its accuracy will interfere with the presence of high levels of specific IgG in the serum of children with milk allergy. To solve this problem, we established a light-initiated chemiluminescent assay (LICA) based on nanomicrospheres, which neutralized the interference of specific IgG by increasing the amount of antigen coated on the microspheres. The ability of this method to resist IgG interference was confirmed by adding extra specific IgG to the serum of allergic patients. Finally, the positive rate of allergen-specific IgE was increased to 85%, which was better than the indirect ELISA (70%), indicating that this method has certain advantages for the detection of specific IgE in children with milk allergy.
过敏原特异性 IgE 的检测对儿童牛奶过敏的诊断具有重要价值。然而，其准确性会受到牛奶过敏患儿血清中高水平特异性 IgG 的干扰。为了解决这个问题，我们建立了一种基于纳米微球的光引发化学发光测定法（LICA），通过增加微球上包被的抗原量来消除特异性 IgG 的干扰。通过向过敏患者的血清中添加额外的特异性 IgG 来验证该方法抵抗 IgG 干扰的能力。最后，过敏原特异性 IgE 的阳性率提高到 85%，优于间接 ELISA（70%），表明该方法在检测牛奶过敏儿童特异性 IgE 方面具有一定优势。
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