Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Int Immunopharmacol. 2021 Oct;99:107898. doi: 10.1016/j.intimp.2021.107898. Epub 2021 Jul 29.
Tuberculosis still threatens human health. We aimed to investigate the T cell immune status and the role of multifunctional T cells in pulmonary tuberculosis patients.
Thirty active pulmonary tuberculosis (APTB) patients, 30 latent tuberculosis infection (LTBI) patients, 25 cured pulmonary tuberculosis (CPTB) patients and 25 healthy controls (HCs) enrolled in this study. Flow cytometer for detecting T cell phenotype and function. CBA Flex Set was used to measure chemokine.
Compared with HCs and LTBI patients, APTB patients had fewer CD4 T and CD8 T cells, but the expression of granzyme A, granzyme B and perforin on CD8 T cells increased. Compared to LTBI and CPTB patients, Mycobacterium tuberculosis-specific CD8 T cells in APTB patients appeared to be more differentiated CD45RACCR7 cells, and there were more multifunctional CD4 T and CD8 T cells. Importantly, the frequency of multifunctional CD4 T cells in the pleural fluid of APTB patients was higher than that of peripheral blood. And the proportion of multifunctional CD4 T cells expressing the migration receptor CXCR3 in the peripheral blood of APTB patients decreased, while the concentrations of its ligands, chemokine MIG, IP-10 and I-TAC increased significantly in plasma, especially in pleural fluid.
Decreased T lymphocytes in APTB patients may cause compensatory activation of CD8 T cells. Multifunctional CD4 T cells in peripheral blood could migrate to the lungs under the action of CXCR3 and associated chemokine. Multifunctional CD4 T cells and Multifunctional CD8 T cells were of great significance in monitoring disease treatment.
结核病仍然威胁着人类健康。我们旨在研究肺结核患者的 T 细胞免疫状态和多功能 T 细胞的作用。
本研究纳入了 30 例活动性肺结核（APTB）患者、30 例潜伏性结核感染（LTBI）患者、25 例治愈性肺结核（CPTB）患者和 25 名健康对照（HCs）。采用流式细胞仪检测 T 细胞表型和功能。CBA Flex Set 用于检测趋化因子。
与 HCs 和 LTBI 患者相比，APTB 患者的 CD4 T 和 CD8 T 细胞减少，但 CD8 T 细胞中颗粒酶 A、颗粒酶 B 和穿孔素的表达增加。与 LTBI 和 CPTB 患者相比，APTB 患者中结核分枝杆菌特异性 CD8 T 细胞似乎是更多分化的 CD45RA+CCR7-细胞，并且存在更多的多功能 CD4 T 和 CD8 T 细胞。重要的是，APTB 患者胸腔积液中多功能 CD4 T 细胞的频率高于外周血。APTB 患者外周血中表达迁移受体 CXCR3 的多功能 CD4 T 细胞比例降低，而其配体趋化因子 MIG、IP-10 和 I-TAC 的浓度在血浆中显著升高，尤其是在胸腔积液中。
APTB 患者 T 淋巴细胞减少可能导致 CD8 T 细胞代偿性激活。外周血中的多功能 CD4 T 细胞在 CXCR3 及相关趋化因子的作用下可迁移至肺部。多功能 CD4 T 细胞和多功能 CD8 T 细胞在监测疾病治疗方面具有重要意义。
